An improved red deer sperm processing protocol is discussed briefly. This protocol contains the steps of the collection, processing and cryopreservation of post mortem collected epididymal spermatozoa of hunted red deer stags. During the roaring season of 2004, epididymal sperm samples of five red deer stags were collected post mortem. Samples were processed in a commercial bovine semen extender, filled into 0.25 ml straws, then they were frozen by holding in liquid N2 vapor. Initial and post-thaw motility was assessed visually. Post-thaw motility was acceptable in case of every stag. This protocol will allow to build a red deer sperm bank by collecting and freezing spermatozoa of the stags hunted in Hungary.
|Number of pages||3|
|Journal||Magyar Allatorvosok Lapja|
|Publication status||Published - dec. 12 2006|
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