Development of a quantitative preclinical screening model for implant osseointegration in rat tail vertebra

Sándor Farkasdi, Dávid Pammer, Róbert Rácz, Gergely Hriczó-Koperdák, Bence Tamás Szabó, Csaba Dobó-Nagy, Beáta Kerémi, József Blazsek, Frederic Cuisinier, Gang Wu, G. Varga

Research output: Article

Abstract

Objectives: Functional tooth replacement and bone regeneration are parts of the daily practice in modern dentistry, but well-reproducible and relatively inexpensive experimental models are still missing. We aimed to develop a new small animal model to monitor osseointegration utilizing the combination of multiple evaluation protocols. Material and methods: After cutting the tail between the C4 and C5 vertebrae in Wistar rats, costume made, parallel walled, non-threaded implants were placed into the center of the tail parallel with its longitudinal axis using a surgical guide. Osseointegration of the titanium implants was followed between 4 and 16 weeks after surgery applying axial extraction force, and resonance frequency analysis as functional tests, and histomorphometry and micro-CT as structural evaluations. Results: In functional tests, we observed that both methods are suitable for the detection of the time-dependent increase in osseointegration, but the sensitivity of the pull-out technique (an approximately five times increase with rather low standard error) was much higher than that of the resonance frequency analysis. In structural evaluations, changes in the detected bone implant contact values measured by histomorphometry (yielding 1.5 times increase, with low variations of data) were more reliable than micro-CT based evaluations to screen the developments of contact between bone and implant. Conclusion: Our results provide evidence that the caudal vertebrae osseointegration model is useful for the preclinical evaluation of implant integration into the bone. Clinical relevance: The combination of the biomechanical and structural tests offers a well-reproducible small animal system that can be suitable for studying the integration of various implant materials and surface treatments.

Original languageEnglish
JournalClinical Oral Investigations
DOIs
Publication statusAccepted/In press - jan. 1 2018

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Osseointegration
Tail
Spine
Bone and Bones
Bone Regeneration
Dentistry
Titanium
Wistar Rats
Tooth
Theoretical Models
Animal Models

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Development of a quantitative preclinical screening model for implant osseointegration in rat tail vertebra. / Farkasdi, Sándor; Pammer, Dávid; Rácz, Róbert; Hriczó-Koperdák, Gergely; Szabó, Bence Tamás; Dobó-Nagy, Csaba; Kerémi, Beáta; Blazsek, József; Cuisinier, Frederic; Wu, Gang; Varga, G.

In: Clinical Oral Investigations, 01.01.2018.

Research output: Article

Farkasdi, S, Pammer, D, Rácz, R, Hriczó-Koperdák, G, Szabó, BT, Dobó-Nagy, C, Kerémi, B, Blazsek, J, Cuisinier, F, Wu, G & Varga, G 2018, 'Development of a quantitative preclinical screening model for implant osseointegration in rat tail vertebra', Clinical Oral Investigations. https://doi.org/10.1007/s00784-018-2661-1
Farkasdi, Sándor ; Pammer, Dávid ; Rácz, Róbert ; Hriczó-Koperdák, Gergely ; Szabó, Bence Tamás ; Dobó-Nagy, Csaba ; Kerémi, Beáta ; Blazsek, József ; Cuisinier, Frederic ; Wu, Gang ; Varga, G. / Development of a quantitative preclinical screening model for implant osseointegration in rat tail vertebra. In: Clinical Oral Investigations. 2018.
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abstract = "Objectives: Functional tooth replacement and bone regeneration are parts of the daily practice in modern dentistry, but well-reproducible and relatively inexpensive experimental models are still missing. We aimed to develop a new small animal model to monitor osseointegration utilizing the combination of multiple evaluation protocols. Material and methods: After cutting the tail between the C4 and C5 vertebrae in Wistar rats, costume made, parallel walled, non-threaded implants were placed into the center of the tail parallel with its longitudinal axis using a surgical guide. Osseointegration of the titanium implants was followed between 4 and 16 weeks after surgery applying axial extraction force, and resonance frequency analysis as functional tests, and histomorphometry and micro-CT as structural evaluations. Results: In functional tests, we observed that both methods are suitable for the detection of the time-dependent increase in osseointegration, but the sensitivity of the pull-out technique (an approximately five times increase with rather low standard error) was much higher than that of the resonance frequency analysis. In structural evaluations, changes in the detected bone implant contact values measured by histomorphometry (yielding 1.5 times increase, with low variations of data) were more reliable than micro-CT based evaluations to screen the developments of contact between bone and implant. Conclusion: Our results provide evidence that the caudal vertebrae osseointegration model is useful for the preclinical evaluation of implant integration into the bone. Clinical relevance: The combination of the biomechanical and structural tests offers a well-reproducible small animal system that can be suitable for studying the integration of various implant materials and surface treatments.",
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AU - Farkasdi, Sándor

AU - Pammer, Dávid

AU - Rácz, Róbert

AU - Hriczó-Koperdák, Gergely

AU - Szabó, Bence Tamás

AU - Dobó-Nagy, Csaba

AU - Kerémi, Beáta

AU - Blazsek, József

AU - Cuisinier, Frederic

AU - Wu, Gang

AU - Varga, G.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Objectives: Functional tooth replacement and bone regeneration are parts of the daily practice in modern dentistry, but well-reproducible and relatively inexpensive experimental models are still missing. We aimed to develop a new small animal model to monitor osseointegration utilizing the combination of multiple evaluation protocols. Material and methods: After cutting the tail between the C4 and C5 vertebrae in Wistar rats, costume made, parallel walled, non-threaded implants were placed into the center of the tail parallel with its longitudinal axis using a surgical guide. Osseointegration of the titanium implants was followed between 4 and 16 weeks after surgery applying axial extraction force, and resonance frequency analysis as functional tests, and histomorphometry and micro-CT as structural evaluations. Results: In functional tests, we observed that both methods are suitable for the detection of the time-dependent increase in osseointegration, but the sensitivity of the pull-out technique (an approximately five times increase with rather low standard error) was much higher than that of the resonance frequency analysis. In structural evaluations, changes in the detected bone implant contact values measured by histomorphometry (yielding 1.5 times increase, with low variations of data) were more reliable than micro-CT based evaluations to screen the developments of contact between bone and implant. Conclusion: Our results provide evidence that the caudal vertebrae osseointegration model is useful for the preclinical evaluation of implant integration into the bone. Clinical relevance: The combination of the biomechanical and structural tests offers a well-reproducible small animal system that can be suitable for studying the integration of various implant materials and surface treatments.

AB - Objectives: Functional tooth replacement and bone regeneration are parts of the daily practice in modern dentistry, but well-reproducible and relatively inexpensive experimental models are still missing. We aimed to develop a new small animal model to monitor osseointegration utilizing the combination of multiple evaluation protocols. Material and methods: After cutting the tail between the C4 and C5 vertebrae in Wistar rats, costume made, parallel walled, non-threaded implants were placed into the center of the tail parallel with its longitudinal axis using a surgical guide. Osseointegration of the titanium implants was followed between 4 and 16 weeks after surgery applying axial extraction force, and resonance frequency analysis as functional tests, and histomorphometry and micro-CT as structural evaluations. Results: In functional tests, we observed that both methods are suitable for the detection of the time-dependent increase in osseointegration, but the sensitivity of the pull-out technique (an approximately five times increase with rather low standard error) was much higher than that of the resonance frequency analysis. In structural evaluations, changes in the detected bone implant contact values measured by histomorphometry (yielding 1.5 times increase, with low variations of data) were more reliable than micro-CT based evaluations to screen the developments of contact between bone and implant. Conclusion: Our results provide evidence that the caudal vertebrae osseointegration model is useful for the preclinical evaluation of implant integration into the bone. Clinical relevance: The combination of the biomechanical and structural tests offers a well-reproducible small animal system that can be suitable for studying the integration of various implant materials and surface treatments.

KW - Histomorphometry

KW - Implant

KW - Micro-CT

KW - Osseointegration

KW - Pull-out test

KW - Resonance frequency analysis

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