The speciation of selenocystine (SeCys), elenomethionine (SeMet), selenoethionine (SeEt) and Se(IV) was performed by on-line coupling of high performance liquid chromatography (HPLC) and atomic fluorescence spectrometry (AFS) with hydride generation (HG) as high efficiency sample introduction system. Reversed-phase liquid chromatography was applied to separate the selenium species with 10 mM ammonium-acetate buffer eluent adjusted to pH 4. The operating conditions for chromatographic separation (gradient elution, run time) and hydride generation (concentration of HCl and NaBH4 solution, argon and hydrogen flow rates) were carefully optimized. The efficiency of hydride generation for all of the four species was determined. Detection limits (DLs) obtained for SeCys, SeMet, SeEt and Se(IV) are 18, 70, 96, and 16 μg dm-3 respectively. The analytical performance of the method was controlled by measuring the selenium content of spiked selenium food supplements. The recoveries were in the range 90-110%.
|Translated title of the contribution||Development of a method for the determination of selenoamino acids and SeIV by direct hydride generation|
|Number of pages||5|
|Journal||Magyar Kemiai Folyoirat, Kemiai Kozlemenyek|
|Publication status||Published - dec. 1 2001|
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