Development of a direct-binding chloramphenicol sensor based on thiol or sulfide mediated self-assembled antibody monolayers

In Seon Park, Dong Kyung Kim, Nora Adanyi, Maria Varadi, Namsoo Kim

Research output: Article

76 Citations (Scopus)

Abstract

A batch-type antibody-immobilized quartz crystal microbalance (QCM) system for detecting chloramphenicol (CAP) was developed. To bind an anti-CAP antibody onto the gold electrode surface of piezoelectric crystals, self-assembled monolayers (SAMs) of different thiols or sulfides were formed by a chemisorption procedure. Then, the anti-CAP antibody was covalently linked to the pre-formed monolayers by an activation procedure using 1-ethyl-3-(3- dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysulfosuccinimide. The antibody-immobilized QCM chip thus prepared was installed in a well holder and was measured for sensor response. Compared with the bare QCM chip and the QCM chip only coated with 3-mercaptopropionic acid (MPA), the antibody-immobilized sensor showed greatly enhanced frequency shifts by 10-50-fold after CAP injection. In this case, CAP detection which was indicated by steady-state resonant frequency shift was accomplished within 10 min. When CAP solution was injected into the reaction cell in 50 mM concentration, the frequency shifts obtained were, respectively, 530 and 505 Hz in case of thiosalicylic acid and MPA immobilization. Repeated use of the sensor chips up to eight times was possible after 1 min regeneration with 0.1 M NaOH. This system demonstrated a potential application of thiol or sulfide mediated SAMs as the pre-coatings of a real-time detection on CAP in solution.

Original languageEnglish
Pages (from-to)667-674
Number of pages8
JournalBiosensors and Bioelectronics
Volume19
Issue number7
DOIs
Publication statusPublished - febr. 15 2004

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Sulfides
Chloramphenicol
Sulfhydryl Compounds
Antibodies
Quartz crystal microbalances
Monolayers
Quartz Crystal Microbalance Techniques
Immobilized Antibodies
Sensors
3-Mercaptopropionic Acid
Self assembled monolayers
Acids
Anti-Idiotypic Antibodies
Ethyldimethylaminopropyl Carbodiimide
Chemisorption
Natural frequencies
Gold
Chemical activation
Immobilization
Coatings

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

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title = "Development of a direct-binding chloramphenicol sensor based on thiol or sulfide mediated self-assembled antibody monolayers",
abstract = "A batch-type antibody-immobilized quartz crystal microbalance (QCM) system for detecting chloramphenicol (CAP) was developed. To bind an anti-CAP antibody onto the gold electrode surface of piezoelectric crystals, self-assembled monolayers (SAMs) of different thiols or sulfides were formed by a chemisorption procedure. Then, the anti-CAP antibody was covalently linked to the pre-formed monolayers by an activation procedure using 1-ethyl-3-(3- dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysulfosuccinimide. The antibody-immobilized QCM chip thus prepared was installed in a well holder and was measured for sensor response. Compared with the bare QCM chip and the QCM chip only coated with 3-mercaptopropionic acid (MPA), the antibody-immobilized sensor showed greatly enhanced frequency shifts by 10-50-fold after CAP injection. In this case, CAP detection which was indicated by steady-state resonant frequency shift was accomplished within 10 min. When CAP solution was injected into the reaction cell in 50 mM concentration, the frequency shifts obtained were, respectively, 530 and 505 Hz in case of thiosalicylic acid and MPA immobilization. Repeated use of the sensor chips up to eight times was possible after 1 min regeneration with 0.1 M NaOH. This system demonstrated a potential application of thiol or sulfide mediated SAMs as the pre-coatings of a real-time detection on CAP in solution.",
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T1 - Development of a direct-binding chloramphenicol sensor based on thiol or sulfide mediated self-assembled antibody monolayers

AU - Park, In Seon

AU - Kim, Dong Kyung

AU - Adanyi, Nora

AU - Varadi, Maria

AU - Kim, Namsoo

PY - 2004/2/15

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N2 - A batch-type antibody-immobilized quartz crystal microbalance (QCM) system for detecting chloramphenicol (CAP) was developed. To bind an anti-CAP antibody onto the gold electrode surface of piezoelectric crystals, self-assembled monolayers (SAMs) of different thiols or sulfides were formed by a chemisorption procedure. Then, the anti-CAP antibody was covalently linked to the pre-formed monolayers by an activation procedure using 1-ethyl-3-(3- dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysulfosuccinimide. The antibody-immobilized QCM chip thus prepared was installed in a well holder and was measured for sensor response. Compared with the bare QCM chip and the QCM chip only coated with 3-mercaptopropionic acid (MPA), the antibody-immobilized sensor showed greatly enhanced frequency shifts by 10-50-fold after CAP injection. In this case, CAP detection which was indicated by steady-state resonant frequency shift was accomplished within 10 min. When CAP solution was injected into the reaction cell in 50 mM concentration, the frequency shifts obtained were, respectively, 530 and 505 Hz in case of thiosalicylic acid and MPA immobilization. Repeated use of the sensor chips up to eight times was possible after 1 min regeneration with 0.1 M NaOH. This system demonstrated a potential application of thiol or sulfide mediated SAMs as the pre-coatings of a real-time detection on CAP in solution.

AB - A batch-type antibody-immobilized quartz crystal microbalance (QCM) system for detecting chloramphenicol (CAP) was developed. To bind an anti-CAP antibody onto the gold electrode surface of piezoelectric crystals, self-assembled monolayers (SAMs) of different thiols or sulfides were formed by a chemisorption procedure. Then, the anti-CAP antibody was covalently linked to the pre-formed monolayers by an activation procedure using 1-ethyl-3-(3- dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysulfosuccinimide. The antibody-immobilized QCM chip thus prepared was installed in a well holder and was measured for sensor response. Compared with the bare QCM chip and the QCM chip only coated with 3-mercaptopropionic acid (MPA), the antibody-immobilized sensor showed greatly enhanced frequency shifts by 10-50-fold after CAP injection. In this case, CAP detection which was indicated by steady-state resonant frequency shift was accomplished within 10 min. When CAP solution was injected into the reaction cell in 50 mM concentration, the frequency shifts obtained were, respectively, 530 and 505 Hz in case of thiosalicylic acid and MPA immobilization. Repeated use of the sensor chips up to eight times was possible after 1 min regeneration with 0.1 M NaOH. This system demonstrated a potential application of thiol or sulfide mediated SAMs as the pre-coatings of a real-time detection on CAP in solution.

KW - Chloramphenicol sensor

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