Development of a Catalase-Based Amperometric Biosensor for the Determination of Increased Catalase Content in Milk Samples

Péter Futo, Gabriella Markus, Attila Kiss, N. Adányi

Research output: Article

12 Citations (Scopus)

Abstract

The aim of our work was to develop a selective amperometric biosensor for infected milk detection. The catalase enzyme (EC 1.11.1.6) was immobilized onto the surface of a thin-layer enzyme cell. The measurements were carried out in stopped-flow injection analysis system (0.8mLmin -1 flow rate, +590mV polarization potential) in organic phase (6% sodium acetate buffer 200mM pH6.0, ferrocene conductive salt (7.5mgL -1) dissolved in acetonitrile. A certain amount of hydrogen peroxide was degraded by the catalase content of the milk. The degradation of the hydrogen peroxide is proportional to the infection of the milk samples.

Original languageEnglish
Pages (from-to)107-113
Number of pages7
JournalElectroanalysis
Volume24
Issue number1
DOIs
Publication statusPublished - jan. 2012

Fingerprint

Biosensors
Catalase
Hydrogen peroxide
Hydrogen Peroxide
Enzymes
Sodium Acetate
Acetonitrile
Buffers
Salts
Sodium
Flow rate
Polarization
Degradation
Milk

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Cite this

Development of a Catalase-Based Amperometric Biosensor for the Determination of Increased Catalase Content in Milk Samples. / Futo, Péter; Markus, Gabriella; Kiss, Attila; Adányi, N.

In: Electroanalysis, Vol. 24, No. 1, 01.2012, p. 107-113.

Research output: Article

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