Development and characterization of ultra-porous silica films made by the sol-gel method. Application to biosensing

Caroline Desfours, Sylvie Calas-Etienne, Robert Horvath, Marta Martin, Csilla Gergely, Frédéric Cuisinier, Pascal Etienne

Research output: Article


The aim of this work is to demonstrate the sensing ability of reverse-symmetry waveguides to investigate adsorption of casein and build-up of poly-L-lysine mediated casein multilayers. A first part of this study is dedicated to the elaboration and characterization of ultra-porous thin films with very low refractive indices by an appropriate sol-gel method. This will form the basis of our planar optical sensors. Optical waveguide light mode spectroscopy is a real-time and sensitive method to study protein adsorption kinetics and lipid bilayers. We used it to test the obtained waveguides for in-situ monitoring of biomolecule adsorption. As a result, significant changes in the incoupling peak position were observed during the layer-by-layer adsorption. Finally, refractive index and thickness of the adsorbed layers were established.

Original languageEnglish
Pages (from-to)435-443
Number of pages9
JournalApplied Physics A: Materials Science and Processing
Issue number2
Publication statusPublished - febr. 1 2014


ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)

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