Determination of paromomycin residues in turkey tissues by liquid chromatography/mass spectrometry

K. Róna, G. Klausz, E. Keller, M. Szakay, P. Laczay, M. Shem-Tov, P. Székely-Körmöczy

Research output: Article

4 Citations (Scopus)


A liquid chromatography-electrospray-mass spectrometry method has been developed and validated for the determination of paromomycin in turkey muscle, liver and kidney, using kanamycin as internal standard. The method consists of solid-phase extractions on mixed-mode columns. The chromatographic separation was carried out on a C18 column using binary gradient elution containing acetonitrile and 5 mM pentafluoropropionic-acid in water. The method was evaluated for specificity, linearity, recovery, accuracy, limit of detection, limit of quantification, intra- and inter-day repeatability, and stability. It was proven to be selective, linear, precise and accurate over the concentration range tested (0.5 × MRL-2 × MRL for each tissue) with correlation coefficients >0.990. The method was successfully used for the residue determination of PARO from edible tissues of turkeys.

Original languageEnglish
Pages (from-to)3792-3798
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Issue number30
Publication statusPublished - nov. 15 2009


ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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