Determination of L-Lactic Acid Content in Foods by Enzyme-Based Amperometric Bioreactor

Zsuzsanna Bori, Gábor Csiffáry, Diána Virág, Marianna Tóth-Markus, Attila Kiss, Nóra Adányi

Research output: Article

6 Citations (Scopus)

Abstract

A thermostable lactate oxidase (from Aerococcus viridans) based bioreactor was elaborated for L-lactate determination in dairy products. The enzyme was immobilised in a thin-layer enzyme cell connected into a flow injection system with an amperometric detector. The linear measuring range was 0.1-1mmol/L L-lactate (R2=0.99; linear regression equation: y=81.2x+3.3). The detection limit was 0.05mmol/L. 40 Samples were analyzed per hour. Considering reproducibility: the signals measured were very stable for over 800 injections, after this period the activity of the cell decreased slowly. The method was used for the control of dairy products and for monitoring milk freshness.

Original languageEnglish
Pages (from-to)158-164
Number of pages7
JournalElectroanalysis
Volume24
Issue number1
DOIs
Publication statusPublished - jan. 2012

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Fingerprint Dive into the research topics of 'Determination of L-Lactic Acid Content in Foods by Enzyme-Based Amperometric Bioreactor'. Together they form a unique fingerprint.

  • Cite this