Determination of dose and time dependence of muscle contractures elicited by caffeine

L. Konya, I. Jóna, A. Kover, F. Aradi

Research output: Article

1 Citation (Scopus)

Abstract

Caffeine contractures were registered on fibre bundle preparations of frog m. semitendinosus. The drug was applied to muscles in normal Ringer for 30 min in concentrations of 3-40 mM. Biphasic contractures were found in all cases above mechanical threshold concentration of caffeine (2.35 mM. calculated value). Parameter values belonging to the two contracture phases (amplitude, delta l1 l2; time, t1 t2) were analysed by computer programmes in correlation with caffeine concentration. It was found that 1. both contracture phases have the same concentration threshold of caffeine; 2. a caffeine-binding site with K(d)=24.6 mM can be supposed in the surface membrane being responsible for the induction of the first contracture phase of caffeine in the muscle; 3. caffeine has a concentration-dependent action on the surface membrane binding site with a tendency of muscles to contract to zero length at infinite caffeine concentration; 4. the speed of caffeine action is practically equal to the solution exchange emphasizing the action site at the surface membrane of the muscle (sarcolemma and T-tubule membrane); 5. the second caffeine contracture phase develops with maximal amplitude above contracture threshold concentration, during which muscles contract to about 40% of their slack length; this corresponds to a sarcomere length less than that of the myosin filament, consequently, a shortening of this filament must also take plase; 6. the concentration dependence of the second contracture phase results in a monotonic decrease of t2 with increasing caffeine concentration. A possibility for the mechanism of caffeine action through binding to proteins involved in the regulation of intracellular calcium level is discussed.

Original languageEnglish
Pages (from-to)129-137
Number of pages9
JournalActa Biologica Academiae Scientiarum Hungaricae
Volume29
Issue number2
Publication statusPublished - 1979

Fingerprint

contracture
Contracture
caffeine
Caffeine
Muscle
muscle
Muscles
muscles
membrane
dosage
Membranes
frog
drug
calcium
dose
binding sites
software
Binding Sites
protein
Sarcolemma

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Determination of dose and time dependence of muscle contractures elicited by caffeine. / Konya, L.; Jóna, I.; Kover, A.; Aradi, F.

In: Acta Biologica Academiae Scientiarum Hungaricae, Vol. 29, No. 2, 1979, p. 129-137.

Research output: Article

@article{0ab2a9bf436e43cc8efa4c581ca4ff30,
title = "Determination of dose and time dependence of muscle contractures elicited by caffeine",
abstract = "Caffeine contractures were registered on fibre bundle preparations of frog m. semitendinosus. The drug was applied to muscles in normal Ringer for 30 min in concentrations of 3-40 mM. Biphasic contractures were found in all cases above mechanical threshold concentration of caffeine (2.35 mM. calculated value). Parameter values belonging to the two contracture phases (amplitude, delta l1 l2; time, t1 t2) were analysed by computer programmes in correlation with caffeine concentration. It was found that 1. both contracture phases have the same concentration threshold of caffeine; 2. a caffeine-binding site with K(d)=24.6 mM can be supposed in the surface membrane being responsible for the induction of the first contracture phase of caffeine in the muscle; 3. caffeine has a concentration-dependent action on the surface membrane binding site with a tendency of muscles to contract to zero length at infinite caffeine concentration; 4. the speed of caffeine action is practically equal to the solution exchange emphasizing the action site at the surface membrane of the muscle (sarcolemma and T-tubule membrane); 5. the second caffeine contracture phase develops with maximal amplitude above contracture threshold concentration, during which muscles contract to about 40{\%} of their slack length; this corresponds to a sarcomere length less than that of the myosin filament, consequently, a shortening of this filament must also take plase; 6. the concentration dependence of the second contracture phase results in a monotonic decrease of t2 with increasing caffeine concentration. A possibility for the mechanism of caffeine action through binding to proteins involved in the regulation of intracellular calcium level is discussed.",
author = "L. Konya and I. J{\'o}na and A. Kover and F. Aradi",
year = "1979",
language = "English",
volume = "29",
pages = "129--137",
journal = "Acta Biologica Hungarica",
issn = "0236-5383",
publisher = "Akademiai Kiado",
number = "2",

}

TY - JOUR

T1 - Determination of dose and time dependence of muscle contractures elicited by caffeine

AU - Konya, L.

AU - Jóna, I.

AU - Kover, A.

AU - Aradi, F.

PY - 1979

Y1 - 1979

N2 - Caffeine contractures were registered on fibre bundle preparations of frog m. semitendinosus. The drug was applied to muscles in normal Ringer for 30 min in concentrations of 3-40 mM. Biphasic contractures were found in all cases above mechanical threshold concentration of caffeine (2.35 mM. calculated value). Parameter values belonging to the two contracture phases (amplitude, delta l1 l2; time, t1 t2) were analysed by computer programmes in correlation with caffeine concentration. It was found that 1. both contracture phases have the same concentration threshold of caffeine; 2. a caffeine-binding site with K(d)=24.6 mM can be supposed in the surface membrane being responsible for the induction of the first contracture phase of caffeine in the muscle; 3. caffeine has a concentration-dependent action on the surface membrane binding site with a tendency of muscles to contract to zero length at infinite caffeine concentration; 4. the speed of caffeine action is practically equal to the solution exchange emphasizing the action site at the surface membrane of the muscle (sarcolemma and T-tubule membrane); 5. the second caffeine contracture phase develops with maximal amplitude above contracture threshold concentration, during which muscles contract to about 40% of their slack length; this corresponds to a sarcomere length less than that of the myosin filament, consequently, a shortening of this filament must also take plase; 6. the concentration dependence of the second contracture phase results in a monotonic decrease of t2 with increasing caffeine concentration. A possibility for the mechanism of caffeine action through binding to proteins involved in the regulation of intracellular calcium level is discussed.

AB - Caffeine contractures were registered on fibre bundle preparations of frog m. semitendinosus. The drug was applied to muscles in normal Ringer for 30 min in concentrations of 3-40 mM. Biphasic contractures were found in all cases above mechanical threshold concentration of caffeine (2.35 mM. calculated value). Parameter values belonging to the two contracture phases (amplitude, delta l1 l2; time, t1 t2) were analysed by computer programmes in correlation with caffeine concentration. It was found that 1. both contracture phases have the same concentration threshold of caffeine; 2. a caffeine-binding site with K(d)=24.6 mM can be supposed in the surface membrane being responsible for the induction of the first contracture phase of caffeine in the muscle; 3. caffeine has a concentration-dependent action on the surface membrane binding site with a tendency of muscles to contract to zero length at infinite caffeine concentration; 4. the speed of caffeine action is practically equal to the solution exchange emphasizing the action site at the surface membrane of the muscle (sarcolemma and T-tubule membrane); 5. the second caffeine contracture phase develops with maximal amplitude above contracture threshold concentration, during which muscles contract to about 40% of their slack length; this corresponds to a sarcomere length less than that of the myosin filament, consequently, a shortening of this filament must also take plase; 6. the concentration dependence of the second contracture phase results in a monotonic decrease of t2 with increasing caffeine concentration. A possibility for the mechanism of caffeine action through binding to proteins involved in the regulation of intracellular calcium level is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0018750549&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018750549&partnerID=8YFLogxK

M3 - Article

C2 - 314214

AN - SCOPUS:0018750549

VL - 29

SP - 129

EP - 137

JO - Acta Biologica Hungarica

JF - Acta Biologica Hungarica

SN - 0236-5383

IS - 2

ER -