Determination of aplovincaminic acid in biological samples by high-performance liquid chromatography

Maria Kozma, Piroska Pudleiner, L. Vereczkey

Research output: Article

13 Citations (Scopus)

Abstract

A method is described for measuring the concentrations of apovincaminic acid and vincaminic acid (internal standard) in blood plasma and urine by high-performance liquid chromatography using ion pair extraction. The main metabolite of vinpocetine, apovincaminic acid, and vincaminic acid were extracted form 1 ml of plasma and urine into chloroform as an ion pair using tetrabutylammonium hydroxide as counter ion. Analysis was carried out on a reversed-phase column of RP-8 with acetonitrile-0.0075 M phosphate buffer (28:72) at pH 3.5 as mobile phase. The eluted derivatives were detected by UV absorption at 254 nm. The sensitivity of the method is 20 ng/ml for AVA in plasma and urine samples. The relative recovery of these compounds added to plasma was about 50%.

Original languageEnglish
Pages (from-to)177-182
Number of pages6
JournalJournal of Chromatography A
Volume241
Issue number1
DOIs
Publication statusPublished - máj. 28 1982

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High performance liquid chromatography
High Pressure Liquid Chromatography
Plasmas
Acids
vinpocetine
Urine
Ions
Radiation counters
Chloroform
Metabolites
Buffers
Blood
Phosphates
Derivatives
Recovery
apovincaminic acid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Determination of aplovincaminic acid in biological samples by high-performance liquid chromatography. / Kozma, Maria; Pudleiner, Piroska; Vereczkey, L.

In: Journal of Chromatography A, Vol. 241, No. 1, 28.05.1982, p. 177-182.

Research output: Article

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