Detection of microRNAs by Northern blot analyses using LNA probes

Research output: Article

65 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are small, endogenous, non-coding RNA species, about 21 nucleotides in length, which modulate the expression of animal and plant target genes at the post-transcriptional level. It has been shown that miRNA based gene regulation plays a pivotal role in pathways involved in growth and development. Understanding miRNA mediated processes requires new technologies enabling efficient detection of small RNA species. Here we report the optimization of a miRNA Northern blot detection method based on LNA (locked nucleic acid)-modified oligonucleotide hybridization. This technique allows sensitive and highly specific detection of mature miRNAs.

Original languageEnglish
Pages (from-to)140-145
Number of pages6
JournalMethods
Volume43
Issue number2
DOIs
Publication statusPublished - okt. 1 2007

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ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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