Detection of early membrane and nuclear alterations of thymocytes upon in vitro ionizing irradiation.

T. Kubasova, A. B. Chukhlovin, Z. Somosy, S. D. Ivanov, G. J. Köteles, E. A. Zherbin, K. P. Hanson

Research output: Article

4 Citations (Scopus)

Abstract

Some membrane and nuclear parameters of rat thymocytes were studied after in vitro X- or gamma-irradiation with doses of 0.5, 1, 2 and 4 Gy followed by incubation for 0.5 to 4 hours at 21-22 degrees C. Early (within the first 2 hours) distinct functional changes of plasma membranes, i.e. increase in Con A binding, autologous rosette-forming capacity, Alcian Blue-induced agglutination, and a decrease in amount of surface negative charges were observed. Meanwhile, the doses applied did not influence the DNA content, and the proportion of pyknotic nuclei did not grossly differ from that of the time-matched controls. However, an increase in AT-rich DNA component was noted. The radiation-induced changes proved to be transient and dose-dependent. In the whole cell populations no irreversible, death-associated events could be detected under the given experimental conditions.

Original languageEnglish
Pages (from-to)277-288
Number of pages12
JournalActa Physiologica Hungarica
Volume81
Issue number3
Publication statusPublished - 1993

Fingerprint

Nuclear Envelope
Thymocytes
Alcian Blue
DNA
Agglutination
Cell Membrane
Radiation
Population
In Vitro Techniques

ASJC Scopus subject areas

  • Physiology

Cite this

Kubasova, T., Chukhlovin, A. B., Somosy, Z., Ivanov, S. D., Köteles, G. J., Zherbin, E. A., & Hanson, K. P. (1993). Detection of early membrane and nuclear alterations of thymocytes upon in vitro ionizing irradiation. Acta Physiologica Hungarica, 81(3), 277-288.

Detection of early membrane and nuclear alterations of thymocytes upon in vitro ionizing irradiation. / Kubasova, T.; Chukhlovin, A. B.; Somosy, Z.; Ivanov, S. D.; Köteles, G. J.; Zherbin, E. A.; Hanson, K. P.

In: Acta Physiologica Hungarica, Vol. 81, No. 3, 1993, p. 277-288.

Research output: Article

Kubasova, T, Chukhlovin, AB, Somosy, Z, Ivanov, SD, Köteles, GJ, Zherbin, EA & Hanson, KP 1993, 'Detection of early membrane and nuclear alterations of thymocytes upon in vitro ionizing irradiation.', Acta Physiologica Hungarica, vol. 81, no. 3, pp. 277-288.
Kubasova, T. ; Chukhlovin, A. B. ; Somosy, Z. ; Ivanov, S. D. ; Köteles, G. J. ; Zherbin, E. A. ; Hanson, K. P. / Detection of early membrane and nuclear alterations of thymocytes upon in vitro ionizing irradiation. In: Acta Physiologica Hungarica. 1993 ; Vol. 81, No. 3. pp. 277-288.
@article{58c1b00cc8e9493cb19d2cddf2c7d3a6,
title = "Detection of early membrane and nuclear alterations of thymocytes upon in vitro ionizing irradiation.",
abstract = "Some membrane and nuclear parameters of rat thymocytes were studied after in vitro X- or gamma-irradiation with doses of 0.5, 1, 2 and 4 Gy followed by incubation for 0.5 to 4 hours at 21-22 degrees C. Early (within the first 2 hours) distinct functional changes of plasma membranes, i.e. increase in Con A binding, autologous rosette-forming capacity, Alcian Blue-induced agglutination, and a decrease in amount of surface negative charges were observed. Meanwhile, the doses applied did not influence the DNA content, and the proportion of pyknotic nuclei did not grossly differ from that of the time-matched controls. However, an increase in AT-rich DNA component was noted. The radiation-induced changes proved to be transient and dose-dependent. In the whole cell populations no irreversible, death-associated events could be detected under the given experimental conditions.",
author = "T. Kubasova and Chukhlovin, {A. B.} and Z. Somosy and Ivanov, {S. D.} and K{\"o}teles, {G. J.} and Zherbin, {E. A.} and Hanson, {K. P.}",
year = "1993",
language = "English",
volume = "81",
pages = "277--288",
journal = "Physiology International",
issn = "2498-602X",
publisher = "Akademiai Kiado",
number = "3",

}

TY - JOUR

T1 - Detection of early membrane and nuclear alterations of thymocytes upon in vitro ionizing irradiation.

AU - Kubasova, T.

AU - Chukhlovin, A. B.

AU - Somosy, Z.

AU - Ivanov, S. D.

AU - Köteles, G. J.

AU - Zherbin, E. A.

AU - Hanson, K. P.

PY - 1993

Y1 - 1993

N2 - Some membrane and nuclear parameters of rat thymocytes were studied after in vitro X- or gamma-irradiation with doses of 0.5, 1, 2 and 4 Gy followed by incubation for 0.5 to 4 hours at 21-22 degrees C. Early (within the first 2 hours) distinct functional changes of plasma membranes, i.e. increase in Con A binding, autologous rosette-forming capacity, Alcian Blue-induced agglutination, and a decrease in amount of surface negative charges were observed. Meanwhile, the doses applied did not influence the DNA content, and the proportion of pyknotic nuclei did not grossly differ from that of the time-matched controls. However, an increase in AT-rich DNA component was noted. The radiation-induced changes proved to be transient and dose-dependent. In the whole cell populations no irreversible, death-associated events could be detected under the given experimental conditions.

AB - Some membrane and nuclear parameters of rat thymocytes were studied after in vitro X- or gamma-irradiation with doses of 0.5, 1, 2 and 4 Gy followed by incubation for 0.5 to 4 hours at 21-22 degrees C. Early (within the first 2 hours) distinct functional changes of plasma membranes, i.e. increase in Con A binding, autologous rosette-forming capacity, Alcian Blue-induced agglutination, and a decrease in amount of surface negative charges were observed. Meanwhile, the doses applied did not influence the DNA content, and the proportion of pyknotic nuclei did not grossly differ from that of the time-matched controls. However, an increase in AT-rich DNA component was noted. The radiation-induced changes proved to be transient and dose-dependent. In the whole cell populations no irreversible, death-associated events could be detected under the given experimental conditions.

UR - http://www.scopus.com/inward/record.url?scp=0027875952&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027875952&partnerID=8YFLogxK

M3 - Article

C2 - 8197883

AN - SCOPUS:0027875952

VL - 81

SP - 277

EP - 288

JO - Physiology International

JF - Physiology International

SN - 2498-602X

IS - 3

ER -