Detection of a mammalian-like astrovirus in bird, European roller (Coracias garrulus)

Péter Pankovics, Ákos Boros, Tamás Kiss, Eric Delwart, Gábor Reuter

Research output: Article

18 Citations (Scopus)

Abstract

Astroviruses are small, non-enveloped viruses with positive sense, single-stranded RNA genomes. The family Astroviridae contains two genera, Mamastrovirus and Avastrovirus, which - based upon our current knowledge - infect mammals and birds, respectively. However, recent seroprevalence study indicated that people with contact to turkeys can develop serological responses to the turkey astrovirus and minks might have been infected with the avastrovirus. These data suggest that the "host species/astrovirus genus" association should be permeable; however, mamastrovirus infection has not been reported from avian species, yet. In this study, a novel astrovirus was identified by viral metagenomics and RT-PCR methods in 2 (11%) out of 19 faecal samples collected from a wild, carnivorous bird species, European rollers (. Coracias garrulus) from two breeding territories in Hungary. The complete genome sequence of astrovirus Er/SZAL6/HUN/2011 (KP663426) was 7025. nt-long and had some unique genomic features including an unusually long spacer between the subgenomic RNA promoter and the ORF2 initiation codon. Using the BLASTp Er/SZAL6/HUN/2011 had the highest aa identities 35%, 61% and 34% to MAstV 32 (JF713710, host: porcine), to MAstV 23 (JF729316, host: rabbit) and to unclassified porcine astrovirus (JX684071) in ORF1a, ORF1b and ORF2, respectively. The same proteins of Er/SZAL6/HUN/2011 had 25%, 66% and 33% aa identities to the corresponding proteins of murine astrovirus (JX544743) as the closest strain. The sequence- and phylogenetic analysis indicated that Er/SZAL6/HUN/2011 represents the first member of a novel mamastrovirus species. Data suggest that both mammals and birds could have been exposed to mamastroviruses and avastroviruses providing opportunities for cross-species infection and viral adaptation with cross-class astroviruses especially in carnivorous animals. Further investigation is needed to determine the origin, natural host species spectrum, distribution and spread of Er/SZAL6/HUN/2011 among vertebrates.

Original languageEnglish
Pages (from-to)114-121
Number of pages8
JournalInfection, Genetics and Evolution
Volume34
DOIs
Publication statusPublished - aug. 1 2015

Fingerprint

Avastrovirus
Mamastrovirus
Astroviridae
Birds
bird
birds
Mammals
Swine
Genome
RNA
Mink
Metagenomics
mammal
genome
Initiator Codon
Hungary
Seroepidemiologic Studies
Porcine astrovirus
Cross Infection
Turkey astrovirus

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Molecular Biology
  • Microbiology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Detection of a mammalian-like astrovirus in bird, European roller (Coracias garrulus). / Pankovics, Péter; Boros, Ákos; Kiss, Tamás; Delwart, Eric; Reuter, Gábor.

In: Infection, Genetics and Evolution, Vol. 34, 01.08.2015, p. 114-121.

Research output: Article

@article{0a04eb54593d421588170128e3e727d6,
title = "Detection of a mammalian-like astrovirus in bird, European roller (Coracias garrulus)",
abstract = "Astroviruses are small, non-enveloped viruses with positive sense, single-stranded RNA genomes. The family Astroviridae contains two genera, Mamastrovirus and Avastrovirus, which - based upon our current knowledge - infect mammals and birds, respectively. However, recent seroprevalence study indicated that people with contact to turkeys can develop serological responses to the turkey astrovirus and minks might have been infected with the avastrovirus. These data suggest that the {"}host species/astrovirus genus{"} association should be permeable; however, mamastrovirus infection has not been reported from avian species, yet. In this study, a novel astrovirus was identified by viral metagenomics and RT-PCR methods in 2 (11{\%}) out of 19 faecal samples collected from a wild, carnivorous bird species, European rollers (. Coracias garrulus) from two breeding territories in Hungary. The complete genome sequence of astrovirus Er/SZAL6/HUN/2011 (KP663426) was 7025. nt-long and had some unique genomic features including an unusually long spacer between the subgenomic RNA promoter and the ORF2 initiation codon. Using the BLASTp Er/SZAL6/HUN/2011 had the highest aa identities 35{\%}, 61{\%} and 34{\%} to MAstV 32 (JF713710, host: porcine), to MAstV 23 (JF729316, host: rabbit) and to unclassified porcine astrovirus (JX684071) in ORF1a, ORF1b and ORF2, respectively. The same proteins of Er/SZAL6/HUN/2011 had 25{\%}, 66{\%} and 33{\%} aa identities to the corresponding proteins of murine astrovirus (JX544743) as the closest strain. The sequence- and phylogenetic analysis indicated that Er/SZAL6/HUN/2011 represents the first member of a novel mamastrovirus species. Data suggest that both mammals and birds could have been exposed to mamastroviruses and avastroviruses providing opportunities for cross-species infection and viral adaptation with cross-class astroviruses especially in carnivorous animals. Further investigation is needed to determine the origin, natural host species spectrum, distribution and spread of Er/SZAL6/HUN/2011 among vertebrates.",
keywords = "Astrovirus, Avastrovirus, Bird, European roller, Mamastrovirus, Mammal",
author = "P{\'e}ter Pankovics and {\'A}kos Boros and Tam{\'a}s Kiss and Eric Delwart and G{\'a}bor Reuter",
year = "2015",
month = "8",
day = "1",
doi = "10.1016/j.meegid.2015.06.020",
language = "English",
volume = "34",
pages = "114--121",
journal = "Infection, Genetics and Evolution",
issn = "1567-1348",
publisher = "Elsevier",

}

TY - JOUR

T1 - Detection of a mammalian-like astrovirus in bird, European roller (Coracias garrulus)

AU - Pankovics, Péter

AU - Boros, Ákos

AU - Kiss, Tamás

AU - Delwart, Eric

AU - Reuter, Gábor

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Astroviruses are small, non-enveloped viruses with positive sense, single-stranded RNA genomes. The family Astroviridae contains two genera, Mamastrovirus and Avastrovirus, which - based upon our current knowledge - infect mammals and birds, respectively. However, recent seroprevalence study indicated that people with contact to turkeys can develop serological responses to the turkey astrovirus and minks might have been infected with the avastrovirus. These data suggest that the "host species/astrovirus genus" association should be permeable; however, mamastrovirus infection has not been reported from avian species, yet. In this study, a novel astrovirus was identified by viral metagenomics and RT-PCR methods in 2 (11%) out of 19 faecal samples collected from a wild, carnivorous bird species, European rollers (. Coracias garrulus) from two breeding territories in Hungary. The complete genome sequence of astrovirus Er/SZAL6/HUN/2011 (KP663426) was 7025. nt-long and had some unique genomic features including an unusually long spacer between the subgenomic RNA promoter and the ORF2 initiation codon. Using the BLASTp Er/SZAL6/HUN/2011 had the highest aa identities 35%, 61% and 34% to MAstV 32 (JF713710, host: porcine), to MAstV 23 (JF729316, host: rabbit) and to unclassified porcine astrovirus (JX684071) in ORF1a, ORF1b and ORF2, respectively. The same proteins of Er/SZAL6/HUN/2011 had 25%, 66% and 33% aa identities to the corresponding proteins of murine astrovirus (JX544743) as the closest strain. The sequence- and phylogenetic analysis indicated that Er/SZAL6/HUN/2011 represents the first member of a novel mamastrovirus species. Data suggest that both mammals and birds could have been exposed to mamastroviruses and avastroviruses providing opportunities for cross-species infection and viral adaptation with cross-class astroviruses especially in carnivorous animals. Further investigation is needed to determine the origin, natural host species spectrum, distribution and spread of Er/SZAL6/HUN/2011 among vertebrates.

AB - Astroviruses are small, non-enveloped viruses with positive sense, single-stranded RNA genomes. The family Astroviridae contains two genera, Mamastrovirus and Avastrovirus, which - based upon our current knowledge - infect mammals and birds, respectively. However, recent seroprevalence study indicated that people with contact to turkeys can develop serological responses to the turkey astrovirus and minks might have been infected with the avastrovirus. These data suggest that the "host species/astrovirus genus" association should be permeable; however, mamastrovirus infection has not been reported from avian species, yet. In this study, a novel astrovirus was identified by viral metagenomics and RT-PCR methods in 2 (11%) out of 19 faecal samples collected from a wild, carnivorous bird species, European rollers (. Coracias garrulus) from two breeding territories in Hungary. The complete genome sequence of astrovirus Er/SZAL6/HUN/2011 (KP663426) was 7025. nt-long and had some unique genomic features including an unusually long spacer between the subgenomic RNA promoter and the ORF2 initiation codon. Using the BLASTp Er/SZAL6/HUN/2011 had the highest aa identities 35%, 61% and 34% to MAstV 32 (JF713710, host: porcine), to MAstV 23 (JF729316, host: rabbit) and to unclassified porcine astrovirus (JX684071) in ORF1a, ORF1b and ORF2, respectively. The same proteins of Er/SZAL6/HUN/2011 had 25%, 66% and 33% aa identities to the corresponding proteins of murine astrovirus (JX544743) as the closest strain. The sequence- and phylogenetic analysis indicated that Er/SZAL6/HUN/2011 represents the first member of a novel mamastrovirus species. Data suggest that both mammals and birds could have been exposed to mamastroviruses and avastroviruses providing opportunities for cross-species infection and viral adaptation with cross-class astroviruses especially in carnivorous animals. Further investigation is needed to determine the origin, natural host species spectrum, distribution and spread of Er/SZAL6/HUN/2011 among vertebrates.

KW - Astrovirus

KW - Avastrovirus

KW - Bird

KW - European roller

KW - Mamastrovirus

KW - Mammal

UR - http://www.scopus.com/inward/record.url?scp=84938062209&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938062209&partnerID=8YFLogxK

U2 - 10.1016/j.meegid.2015.06.020

DO - 10.1016/j.meegid.2015.06.020

M3 - Article

C2 - 26096774

AN - SCOPUS:84938062209

VL - 34

SP - 114

EP - 121

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

SN - 1567-1348

ER -