Detection and characterization of artefact compounds during selenium speciation analysis in yeast by ICP-MS-assisted MALDI MS, oMALDI MS/MS and LC-ES-MS/MS

M. Dernovics, L. Ouerdane, L. Tastet, P. Giusti, H. Preud'Homme, R. Lobinski

Research output: Article

14 Citations (Scopus)

Abstract

Analysis of selenopeptides, isolated (and considered as pure) by 2D size-exclusion-reversed-phase HPLC, by capillary HPLC-ICP MS revealed a number of previously unreported Se-containing compounds. Their identity and origin were studied by the state-of-the art molecular mass spectrometric techniques including MALDI MS, oMALDI MS/MS and nanoLC-electrospray QTOF MS/MS. The research showed for the first time the possibility of loss of selenium from selenomethionine by degradation to vinylglycine and the formation of new Se peptides by methylation on carboxylic residues. The study substantiates claims for the use of the highest possible resolution HPLC for speciation studies. It raises concerns about misinterpretation of HPLC-ICP MS data on Se-speciation in selenised yeast since a number of peaks observed may be due to the formation of artefact compounds during some analytical procedures.

Original languageEnglish
Pages (from-to)703-707
Number of pages5
JournalJournal of Analytical Atomic Spectrometry
Volume21
Issue number7
DOIs
Publication statusPublished - 2006

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Selenium compounds
Selenomethionine
Methylation
Molecular mass
Selenium
Yeast
Degradation
Peptides
vinylglycine

ASJC Scopus subject areas

  • Spectroscopy

Cite this

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title = "Detection and characterization of artefact compounds during selenium speciation analysis in yeast by ICP-MS-assisted MALDI MS, oMALDI MS/MS and LC-ES-MS/MS",
abstract = "Analysis of selenopeptides, isolated (and considered as pure) by 2D size-exclusion-reversed-phase HPLC, by capillary HPLC-ICP MS revealed a number of previously unreported Se-containing compounds. Their identity and origin were studied by the state-of-the art molecular mass spectrometric techniques including MALDI MS, oMALDI MS/MS and nanoLC-electrospray QTOF MS/MS. The research showed for the first time the possibility of loss of selenium from selenomethionine by degradation to vinylglycine and the formation of new Se peptides by methylation on carboxylic residues. The study substantiates claims for the use of the highest possible resolution HPLC for speciation studies. It raises concerns about misinterpretation of HPLC-ICP MS data on Se-speciation in selenised yeast since a number of peaks observed may be due to the formation of artefact compounds during some analytical procedures.",
author = "M. Dernovics and L. Ouerdane and L. Tastet and P. Giusti and H. Preud'Homme and R. Lobinski",
year = "2006",
doi = "10.1039/b604885d",
language = "English",
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pages = "703--707",
journal = "Journal of Analytical Atomic Spectrometry",
issn = "0267-9477",
publisher = "Royal Society of Chemistry",
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TY - JOUR

T1 - Detection and characterization of artefact compounds during selenium speciation analysis in yeast by ICP-MS-assisted MALDI MS, oMALDI MS/MS and LC-ES-MS/MS

AU - Dernovics, M.

AU - Ouerdane, L.

AU - Tastet, L.

AU - Giusti, P.

AU - Preud'Homme, H.

AU - Lobinski, R.

PY - 2006

Y1 - 2006

N2 - Analysis of selenopeptides, isolated (and considered as pure) by 2D size-exclusion-reversed-phase HPLC, by capillary HPLC-ICP MS revealed a number of previously unreported Se-containing compounds. Their identity and origin were studied by the state-of-the art molecular mass spectrometric techniques including MALDI MS, oMALDI MS/MS and nanoLC-electrospray QTOF MS/MS. The research showed for the first time the possibility of loss of selenium from selenomethionine by degradation to vinylglycine and the formation of new Se peptides by methylation on carboxylic residues. The study substantiates claims for the use of the highest possible resolution HPLC for speciation studies. It raises concerns about misinterpretation of HPLC-ICP MS data on Se-speciation in selenised yeast since a number of peaks observed may be due to the formation of artefact compounds during some analytical procedures.

AB - Analysis of selenopeptides, isolated (and considered as pure) by 2D size-exclusion-reversed-phase HPLC, by capillary HPLC-ICP MS revealed a number of previously unreported Se-containing compounds. Their identity and origin were studied by the state-of-the art molecular mass spectrometric techniques including MALDI MS, oMALDI MS/MS and nanoLC-electrospray QTOF MS/MS. The research showed for the first time the possibility of loss of selenium from selenomethionine by degradation to vinylglycine and the formation of new Se peptides by methylation on carboxylic residues. The study substantiates claims for the use of the highest possible resolution HPLC for speciation studies. It raises concerns about misinterpretation of HPLC-ICP MS data on Se-speciation in selenised yeast since a number of peaks observed may be due to the formation of artefact compounds during some analytical procedures.

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DO - 10.1039/b604885d

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