Delayed vaccine virus replication in chickens vaccinated subcutaneously with an immune complex infectious bursal disease vaccine: Quantification of vaccine virus by real-time polymerase chain reaction

Judith Iván, Maja Velhner, K. Ursu, Péter Germán, Tamás Mató, Csaba Nick Drén, János Mészáros

Research output: Article

18 Citations (Scopus)

Abstract

The distribution of the immune complex vaccine virus for infectious bursal disease (IBD) in tissue was examined and the viral loads of the organs were quantitatively compared. One-day-old specific pathogen free (SPF) and maternally immune broiler chickens were injected subcutaneously with the vaccine. Lymphoid and non-lymphoid tissues were collected at various time intervals during the experiment to test for infectious bursal disease virus (IBDV)-RNA by using reverse transcriptase-polymerase chain reaction (RT-PCR). Only the bursa of Fabricius was found to be positive with unusually long viral persistence in the broiler group. The positive bursa samples were further investigated by using real-time PCR coupled with a TaqMan probe. The highest amounts of the virus were detected at its first appearance in the bursa: on day 14 post vaccination (PV) in the SPF chickens and on day 17 and day 21 PV in the maternally immune broiler group. The virus then gradually cleared, most likely due to the parallel appearance of the active immune response indicated by seroconversion.

Original languageEnglish
Pages (from-to)135-142
Number of pages8
JournalCanadian Journal of Veterinary Research
Volume69
Issue number2
Publication statusPublished - ápr. 2005

Fingerprint

Infectious bursal disease virus
Specific Pathogen-Free Organisms
infectious bursal disease
antigen-antibody complex
Virus Replication
virus replication
Antigen-Antibody Complex
Communicable Diseases
Real-Time Polymerase Chain Reaction
Chickens
quantitative polymerase chain reaction
Vaccination
Vaccines
broiler chickens
Active Immunity
Bursa of Fabricius
vaccines
chickens
Viruses
viruses

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Delayed vaccine virus replication in chickens vaccinated subcutaneously with an immune complex infectious bursal disease vaccine : Quantification of vaccine virus by real-time polymerase chain reaction. / Iván, Judith; Velhner, Maja; Ursu, K.; Germán, Péter; Mató, Tamás; Drén, Csaba Nick; Mészáros, János.

In: Canadian Journal of Veterinary Research, Vol. 69, No. 2, 04.2005, p. 135-142.

Research output: Article

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