Delayed fluorescence measurements show increased S2Q b charge recombination in PS2 of tobacco pigment-deficient aurea mutant

Detelin Stefanov, Georgi Milanov, P. Lambrev, Maya Kurteva, Nabil Abumhadi, Vasilij Goltsev, Veneta Kapchina

Research output: Article

Abstract

Genetic transformation of plants often results in changed pigment content. Variation in pigment content directly induces changes in photosynthetic electron transport reactions. Fully expanded and deficient in chlorophylls tobacco leaves of aurea mutant, A1146, that expresses enhanced level of QB-nonreducing PS2 centres and increased possibility for charge recombination of separated states in PS2 reaction centres were investigated. Long-term delayed fluorescence, DF, measured in second-to-minute time domains and temperature curve of delayed fluorescence, DFTC, was used to study slow PS2 charge recombination (S2 Q A and S2Q in both wild type (line N1146) and a mutant A1146 tobacco plants. InB)conditions when S2 Q B recombination prevails in PS2 reaction centres, it causes increased possibility for PS2 photodamages. We found that despite of higher possibility for S2 Q B recombination in A1146 as evaluated by DF decay kinetics these plants do not differ from WT in their efficiency for photoprotection. The lack of ‘B-like-band’ in DFTC of leaves of both studied tobacco lines pre-illuminated for 6 min with low light intensities of 45 µmol m−2 s−1 PPFD (LL) was observed. It was considered a manifestation of photoprotection at LL conditions through limiting possibility for S2 Q B recombination. In leaves pre-illuminated for 6 min with high light intensities of 1200 µmol m−2 s−1 PPFD (HL) A1146 half-drop of well-expressed B-band was shifted to higher temperatures. A possible photoprotection based on accumulation of QB-nonreducing centres was discussed in details.

Original languageEnglish
Pages (from-to)1052-1061
Number of pages10
JournalComptes Rendus de L'Academie Bulgare des Sciences
Volume71
Issue number8
DOIs
Publication statusPublished - jan. 1 2018

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photostability
tobacco
pigments
fluorescence
mutants
light intensity
leaves
genetic transformation
electron transfer
temperature
deterioration
chlorophyll
kinetics

ASJC Scopus subject areas

  • General

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Delayed fluorescence measurements show increased S2Q b charge recombination in PS2 of tobacco pigment-deficient aurea mutant. / Stefanov, Detelin; Milanov, Georgi; Lambrev, P.; Kurteva, Maya; Abumhadi, Nabil; Goltsev, Vasilij; Kapchina, Veneta.

In: Comptes Rendus de L'Academie Bulgare des Sciences, Vol. 71, No. 8, 01.01.2018, p. 1052-1061.

Research output: Article

Stefanov, Detelin ; Milanov, Georgi ; Lambrev, P. ; Kurteva, Maya ; Abumhadi, Nabil ; Goltsev, Vasilij ; Kapchina, Veneta. / Delayed fluorescence measurements show increased S2Q b charge recombination in PS2 of tobacco pigment-deficient aurea mutant. In: Comptes Rendus de L'Academie Bulgare des Sciences. 2018 ; Vol. 71, No. 8. pp. 1052-1061.
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abstract = "Genetic transformation of plants often results in changed pigment content. Variation in pigment content directly induces changes in photosynthetic electron transport reactions. Fully expanded and deficient in chlorophylls tobacco leaves of aurea mutant, A1146, that expresses enhanced level of QB-nonreducing PS2 centres and increased possibility for charge recombination of separated states in PS2 reaction centres were investigated. Long-term delayed fluorescence, DF, measured in second-to-minute time domains and temperature curve of delayed fluorescence, DFTC, was used to study slow PS2 charge recombination (S2 Q− A and S2Q− in both wild type (line N1146) and a mutant A1146 tobacco plants. InB)conditions when S2 Q− B recombination prevails in PS2 reaction centres, it causes increased possibility for PS2 photodamages. We found that despite of higher possibility for S2 Q− B recombination in A1146 as evaluated by DF decay kinetics these plants do not differ from WT in their efficiency for photoprotection. The lack of ‘B-like-band’ in DFTC of leaves of both studied tobacco lines pre-illuminated for 6 min with low light intensities of 45 µmol m−2 s−1 PPFD (LL) was observed. It was considered a manifestation of photoprotection at LL conditions through limiting possibility for S2 Q− B recombination. In leaves pre-illuminated for 6 min with high light intensities of 1200 µmol m−2 s−1 PPFD (HL) A1146 half-drop of well-expressed B-band was shifted to higher temperatures. A possible photoprotection based on accumulation of QB-nonreducing centres was discussed in details.",
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AU - Stefanov, Detelin

AU - Milanov, Georgi

AU - Lambrev, P.

AU - Kurteva, Maya

AU - Abumhadi, Nabil

AU - Goltsev, Vasilij

AU - Kapchina, Veneta

PY - 2018/1/1

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N2 - Genetic transformation of plants often results in changed pigment content. Variation in pigment content directly induces changes in photosynthetic electron transport reactions. Fully expanded and deficient in chlorophylls tobacco leaves of aurea mutant, A1146, that expresses enhanced level of QB-nonreducing PS2 centres and increased possibility for charge recombination of separated states in PS2 reaction centres were investigated. Long-term delayed fluorescence, DF, measured in second-to-minute time domains and temperature curve of delayed fluorescence, DFTC, was used to study slow PS2 charge recombination (S2 Q− A and S2Q− in both wild type (line N1146) and a mutant A1146 tobacco plants. InB)conditions when S2 Q− B recombination prevails in PS2 reaction centres, it causes increased possibility for PS2 photodamages. We found that despite of higher possibility for S2 Q− B recombination in A1146 as evaluated by DF decay kinetics these plants do not differ from WT in their efficiency for photoprotection. The lack of ‘B-like-band’ in DFTC of leaves of both studied tobacco lines pre-illuminated for 6 min with low light intensities of 45 µmol m−2 s−1 PPFD (LL) was observed. It was considered a manifestation of photoprotection at LL conditions through limiting possibility for S2 Q− B recombination. In leaves pre-illuminated for 6 min with high light intensities of 1200 µmol m−2 s−1 PPFD (HL) A1146 half-drop of well-expressed B-band was shifted to higher temperatures. A possible photoprotection based on accumulation of QB-nonreducing centres was discussed in details.

AB - Genetic transformation of plants often results in changed pigment content. Variation in pigment content directly induces changes in photosynthetic electron transport reactions. Fully expanded and deficient in chlorophylls tobacco leaves of aurea mutant, A1146, that expresses enhanced level of QB-nonreducing PS2 centres and increased possibility for charge recombination of separated states in PS2 reaction centres were investigated. Long-term delayed fluorescence, DF, measured in second-to-minute time domains and temperature curve of delayed fluorescence, DFTC, was used to study slow PS2 charge recombination (S2 Q− A and S2Q− in both wild type (line N1146) and a mutant A1146 tobacco plants. InB)conditions when S2 Q− B recombination prevails in PS2 reaction centres, it causes increased possibility for PS2 photodamages. We found that despite of higher possibility for S2 Q− B recombination in A1146 as evaluated by DF decay kinetics these plants do not differ from WT in their efficiency for photoprotection. The lack of ‘B-like-band’ in DFTC of leaves of both studied tobacco lines pre-illuminated for 6 min with low light intensities of 45 µmol m−2 s−1 PPFD (LL) was observed. It was considered a manifestation of photoprotection at LL conditions through limiting possibility for S2 Q− B recombination. In leaves pre-illuminated for 6 min with high light intensities of 1200 µmol m−2 s−1 PPFD (HL) A1146 half-drop of well-expressed B-band was shifted to higher temperatures. A possible photoprotection based on accumulation of QB-nonreducing centres was discussed in details.

KW - Chlorophyll deficient varieties

KW - Delayed fluorescence

KW - Delayed fluorescence temperature curves

KW - Photosynthesis

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