Decreased Nuclear Ascorbate Accumulation Accompanied with Altered Genomic Methylation Pattern in Fibroblasts from Arterial Tortuosity Syndrome Patients

Csilla E. Németh, Z. Nemoda, Péter Lőw, P. Szabó, Erzsébet Z. Horváth, Andy Willaert, Annekatrien Boel, Bert L. Callewaert, Paul J. Coucke, Marina Colombi, G. Bánhegyi, E. Margittai

Research output: Article

1 Citation (Scopus)

Abstract

Ascorbate requiring Fe2+/2-oxoglutarate-dependent dioxygenases located in the nucleoplasm have been shown to participate in epigenetic regulation of gene expression via histone and DNA demethylation. Transport of dehydroascorbic acid is impaired in the endomembranes of fibroblasts from arterial tortuosity syndrome (ATS) patients, due to the mutation in the gene coding for glucose transporter GLUT10. We hypothesized that altered nuclear ascorbate concentration might be present in ATS fibroblasts, affecting dioxygenase activity and DNA demethylation. Therefore, our aim was to characterize the subcellular distribution of vitamin C, the global and site-specific changes in 5-methylcytosine and 5-hydroxymethylcytosine levels, and the effect of ascorbate supplementation in control and ATS fibroblast cultures. Diminished nuclear accumulation of ascorbate was found in ATS fibroblasts upon ascorbate or dehydroascorbic acid addition. Analyzing DNA samples of cultured fibroblasts from controls and ATS patients, a lower global 5-hydroxymethylcytosine level was found in ATS fibroblasts, which could not be significantly modified by ascorbate addition. Investigation of the (hydroxy)methylation status of specific regions in six candidate genes related to ascorbate metabolism and function showed that ascorbate addition could stimulate hydroxymethylation and active DNA demethylation at the PPAR-γ gene region in control fibroblasts only. The altered DNA hydroxymethylation patterns in patient cells both at the global level and at specific gene regions accompanied with decreased nuclear accumulation of ascorbate suggests the epigenetic role of vitamin C in the pathomechanism of ATS. The present findings represent the first example for the role of vitamin C transport in epigenetic regulation suggesting that ATS is a compartmentalization disease.

Original languageEnglish
Number of pages1
JournalOxidative medicine and cellular longevity
Volume2019
DOIs
Publication statusPublished - jan. 1 2019

Fingerprint

Methylation
Fibroblasts
Genes
Dehydroascorbic Acid
DNA
Ascorbic Acid
Dioxygenases
Epigenomics
5-Methylcytosine
Peroxisome Proliferator-Activated Receptors
Facilitative Glucose Transport Proteins
Arterial Tortuosity Syndrome
Cell culture
Metabolism
Gene expression
Gene Expression Regulation
Histones
Mutation

ASJC Scopus subject areas

  • Biochemistry
  • Ageing
  • Cell Biology

Cite this

Decreased Nuclear Ascorbate Accumulation Accompanied with Altered Genomic Methylation Pattern in Fibroblasts from Arterial Tortuosity Syndrome Patients. / Németh, Csilla E.; Nemoda, Z.; Lőw, Péter; Szabó, P.; Horváth, Erzsébet Z.; Willaert, Andy; Boel, Annekatrien; Callewaert, Bert L.; Coucke, Paul J.; Colombi, Marina; Bánhegyi, G.; Margittai, E.

In: Oxidative medicine and cellular longevity, Vol. 2019, 01.01.2019.

Research output: Article

Németh, Csilla E. ; Nemoda, Z. ; Lőw, Péter ; Szabó, P. ; Horváth, Erzsébet Z. ; Willaert, Andy ; Boel, Annekatrien ; Callewaert, Bert L. ; Coucke, Paul J. ; Colombi, Marina ; Bánhegyi, G. ; Margittai, E. / Decreased Nuclear Ascorbate Accumulation Accompanied with Altered Genomic Methylation Pattern in Fibroblasts from Arterial Tortuosity Syndrome Patients. In: Oxidative medicine and cellular longevity. 2019 ; Vol. 2019.
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abstract = "Ascorbate requiring Fe2+/2-oxoglutarate-dependent dioxygenases located in the nucleoplasm have been shown to participate in epigenetic regulation of gene expression via histone and DNA demethylation. Transport of dehydroascorbic acid is impaired in the endomembranes of fibroblasts from arterial tortuosity syndrome (ATS) patients, due to the mutation in the gene coding for glucose transporter GLUT10. We hypothesized that altered nuclear ascorbate concentration might be present in ATS fibroblasts, affecting dioxygenase activity and DNA demethylation. Therefore, our aim was to characterize the subcellular distribution of vitamin C, the global and site-specific changes in 5-methylcytosine and 5-hydroxymethylcytosine levels, and the effect of ascorbate supplementation in control and ATS fibroblast cultures. Diminished nuclear accumulation of ascorbate was found in ATS fibroblasts upon ascorbate or dehydroascorbic acid addition. Analyzing DNA samples of cultured fibroblasts from controls and ATS patients, a lower global 5-hydroxymethylcytosine level was found in ATS fibroblasts, which could not be significantly modified by ascorbate addition. Investigation of the (hydroxy)methylation status of specific regions in six candidate genes related to ascorbate metabolism and function showed that ascorbate addition could stimulate hydroxymethylation and active DNA demethylation at the PPAR-γ gene region in control fibroblasts only. The altered DNA hydroxymethylation patterns in patient cells both at the global level and at specific gene regions accompanied with decreased nuclear accumulation of ascorbate suggests the epigenetic role of vitamin C in the pathomechanism of ATS. The present findings represent the first example for the role of vitamin C transport in epigenetic regulation suggesting that ATS is a compartmentalization disease.",
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AU - Nemoda, Z.

AU - Lőw, Péter

AU - Szabó, P.

AU - Horváth, Erzsébet Z.

AU - Willaert, Andy

AU - Boel, Annekatrien

AU - Callewaert, Bert L.

AU - Coucke, Paul J.

AU - Colombi, Marina

AU - Bánhegyi, G.

AU - Margittai, E.

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