Decrease in CD3-negative-CD8dim+ and Vδ2/Vγ9 TcR + peripheral blood lymphocyte counts, low perforin expression and the impairment of natural killer cell activity is associated with chronic hepatitis C virus infection

G. Pár, Daniel Rukavina, Eckhard R. Podack, M. Horányi, J. Szekeres-Barthó, Géza Hegedüs, Mária Paál, L. Szereday, G. Mózsik, A. Pár

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Abstract

Background/Aims: As chronic hepatitis C virus (HCV) infection is associated with impaired natural killer (NK) cell cytotoxicity, we examined the phenotypes and perforin expression of peripheral blood lymphocytes, as well as the effect of interferon-α2b (IFN-α2b) therapy. Methods: Thirty-three patients had chronic hepatitis C, and of them 12 had been on IFN-α2b treatment. Eleven individuals had been treated earlier with IFN-α2b and completely cured, and eight were HCV carriers with persistently normal serum alanine aminotransferase. Three-colour flow cytometry was used to measure the percentage of CD3+/- CD8+, CD3 + CD4+, γδTcR+, Vδ2 TcR +, Vγ9 TcR +, Vδ1 TcR+, CD3 - CD16+, CD3 - CD56+, CD19 + and perforin-positive cells. NK cell activity was assessed by single cell cytotoxic and flow cytometric assay. Results: Patients with chronic hepatitis C showed an impaired NK cytotoxicity, decreased percentage of CD3-negative-CD8dim-positive (NK subtype) and Vγ9/Vδ2 TcR + as well as perforin-positive T lymphocytes, compared to controls and to those who were cured from HCV infection. IFN-α2b increased NK cell cytotoxicity and the percentage of perforin-positive lymphocytes. Conclusions: Our findings suggest that in chronic HCV infection a decreased percentage of CD3-CD8+, Vγ9/Vδ2 TcR + and perforin-positive T cells and simultaneous decreased peripheral NK activity may contribute to the impaired cellular immune response and the chronicity of the disease.

Original languageEnglish
Pages (from-to)514-522
Number of pages9
JournalJournal of Hepatology
Volume37
Issue number4
DOIs
Publication statusPublished - okt. 2002

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Perforin
Lymphocyte Count
Chronic Hepatitis C
Virus Diseases
Natural Killer Cells
Hepacivirus
Interferons
Lymphocytes
T-Lymphocytes
Alanine Transaminase
Cellular Immunity
Flow Cytometry
Color
Phenotype
Therapeutics
Serum

ASJC Scopus subject areas

  • Gastroenterology

Cite this

@article{ab32e37a4512450298f7c9897e9b5eeb,
title = "Decrease in CD3-negative-CD8dim+ and Vδ2/Vγ9 TcR + peripheral blood lymphocyte counts, low perforin expression and the impairment of natural killer cell activity is associated with chronic hepatitis C virus infection",
abstract = "Background/Aims: As chronic hepatitis C virus (HCV) infection is associated with impaired natural killer (NK) cell cytotoxicity, we examined the phenotypes and perforin expression of peripheral blood lymphocytes, as well as the effect of interferon-α2b (IFN-α2b) therapy. Methods: Thirty-three patients had chronic hepatitis C, and of them 12 had been on IFN-α2b treatment. Eleven individuals had been treated earlier with IFN-α2b and completely cured, and eight were HCV carriers with persistently normal serum alanine aminotransferase. Three-colour flow cytometry was used to measure the percentage of CD3+/- CD8+, CD3 + CD4+, γδTcR+, Vδ2 TcR +, Vγ9 TcR +, Vδ1 TcR+, CD3 - CD16+, CD3 - CD56+, CD19 + and perforin-positive cells. NK cell activity was assessed by single cell cytotoxic and flow cytometric assay. Results: Patients with chronic hepatitis C showed an impaired NK cytotoxicity, decreased percentage of CD3-negative-CD8dim-positive (NK subtype) and Vγ9/Vδ2 TcR + as well as perforin-positive T lymphocytes, compared to controls and to those who were cured from HCV infection. IFN-α2b increased NK cell cytotoxicity and the percentage of perforin-positive lymphocytes. Conclusions: Our findings suggest that in chronic HCV infection a decreased percentage of CD3-CD8+, Vγ9/Vδ2 TcR + and perforin-positive T cells and simultaneous decreased peripheral NK activity may contribute to the impaired cellular immune response and the chronicity of the disease.",
keywords = "CD3-negative-CD8 lymphocytes, Gamma/delta T cells, Hepatitis C virus, Interferon, Natural killer cytotoxicity, Perforin",
author = "G. P{\'a}r and Daniel Rukavina and Podack, {Eckhard R.} and M. Hor{\'a}nyi and J. Szekeres-Barth{\'o} and G{\'e}za Heged{\"u}s and M{\'a}ria Pa{\'a}l and L. Szereday and G. M{\'o}zsik and A. P{\'a}r",
year = "2002",
month = "10",
doi = "10.1016/S0168-8278(02)00218-0",
language = "English",
volume = "37",
pages = "514--522",
journal = "Journal of Hepatology",
issn = "0168-8278",
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number = "4",

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TY - JOUR

T1 - Decrease in CD3-negative-CD8dim+ and Vδ2/Vγ9 TcR + peripheral blood lymphocyte counts, low perforin expression and the impairment of natural killer cell activity is associated with chronic hepatitis C virus infection

AU - Pár, G.

AU - Rukavina, Daniel

AU - Podack, Eckhard R.

AU - Horányi, M.

AU - Szekeres-Barthó, J.

AU - Hegedüs, Géza

AU - Paál, Mária

AU - Szereday, L.

AU - Mózsik, G.

AU - Pár, A.

PY - 2002/10

Y1 - 2002/10

N2 - Background/Aims: As chronic hepatitis C virus (HCV) infection is associated with impaired natural killer (NK) cell cytotoxicity, we examined the phenotypes and perforin expression of peripheral blood lymphocytes, as well as the effect of interferon-α2b (IFN-α2b) therapy. Methods: Thirty-three patients had chronic hepatitis C, and of them 12 had been on IFN-α2b treatment. Eleven individuals had been treated earlier with IFN-α2b and completely cured, and eight were HCV carriers with persistently normal serum alanine aminotransferase. Three-colour flow cytometry was used to measure the percentage of CD3+/- CD8+, CD3 + CD4+, γδTcR+, Vδ2 TcR +, Vγ9 TcR +, Vδ1 TcR+, CD3 - CD16+, CD3 - CD56+, CD19 + and perforin-positive cells. NK cell activity was assessed by single cell cytotoxic and flow cytometric assay. Results: Patients with chronic hepatitis C showed an impaired NK cytotoxicity, decreased percentage of CD3-negative-CD8dim-positive (NK subtype) and Vγ9/Vδ2 TcR + as well as perforin-positive T lymphocytes, compared to controls and to those who were cured from HCV infection. IFN-α2b increased NK cell cytotoxicity and the percentage of perforin-positive lymphocytes. Conclusions: Our findings suggest that in chronic HCV infection a decreased percentage of CD3-CD8+, Vγ9/Vδ2 TcR + and perforin-positive T cells and simultaneous decreased peripheral NK activity may contribute to the impaired cellular immune response and the chronicity of the disease.

AB - Background/Aims: As chronic hepatitis C virus (HCV) infection is associated with impaired natural killer (NK) cell cytotoxicity, we examined the phenotypes and perforin expression of peripheral blood lymphocytes, as well as the effect of interferon-α2b (IFN-α2b) therapy. Methods: Thirty-three patients had chronic hepatitis C, and of them 12 had been on IFN-α2b treatment. Eleven individuals had been treated earlier with IFN-α2b and completely cured, and eight were HCV carriers with persistently normal serum alanine aminotransferase. Three-colour flow cytometry was used to measure the percentage of CD3+/- CD8+, CD3 + CD4+, γδTcR+, Vδ2 TcR +, Vγ9 TcR +, Vδ1 TcR+, CD3 - CD16+, CD3 - CD56+, CD19 + and perforin-positive cells. NK cell activity was assessed by single cell cytotoxic and flow cytometric assay. Results: Patients with chronic hepatitis C showed an impaired NK cytotoxicity, decreased percentage of CD3-negative-CD8dim-positive (NK subtype) and Vγ9/Vδ2 TcR + as well as perforin-positive T lymphocytes, compared to controls and to those who were cured from HCV infection. IFN-α2b increased NK cell cytotoxicity and the percentage of perforin-positive lymphocytes. Conclusions: Our findings suggest that in chronic HCV infection a decreased percentage of CD3-CD8+, Vγ9/Vδ2 TcR + and perforin-positive T cells and simultaneous decreased peripheral NK activity may contribute to the impaired cellular immune response and the chronicity of the disease.

KW - CD3-negative-CD8 lymphocytes

KW - Gamma/delta T cells

KW - Hepatitis C virus

KW - Interferon

KW - Natural killer cytotoxicity

KW - Perforin

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U2 - 10.1016/S0168-8278(02)00218-0

DO - 10.1016/S0168-8278(02)00218-0

M3 - Article

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AN - SCOPUS:0036783791

VL - 37

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JO - Journal of Hepatology

JF - Journal of Hepatology

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