Daunomycin-polypeptide conjugates with antitumor activity

Rita Szabó, Z. Bánóczi, G. Mező, Orsolya Láng, L. Kőhidai, F. Hudecz

Research output: Article

10 Citations (Scopus)

Abstract

We have developed a group of water-soluble drug conjugates in which daunomycin (Dau) is coupled to cationic, amphoteric or anionic branched polypeptides and a new conjugate containing a cationic polypeptide carrier modified with a cell penetrating octaarginine. We investigated in vitro physiological activity of these conjugates in several aspects: in vitro cytotoxicity and cytostatic effect, adhesion and cellular uptake were examined on murine (J774 and L1210) and human (MonoMac6 and HL-60) leukemia cell lines and on murine bone marrow derived macrophages. We found that these processes are dependent on the properties of the carrier, on experimental conditions like concentration and incubation time. We found that attachment of polypeptide and cell penetrating peptide to the bioactive agent, depending on the cell line, could significantly improve the antitumor activity of the drug.

Original languageEnglish
Pages (from-to)2209-2216
Number of pages8
JournalBBA - Biomembranes
Volume1798
Issue number12
DOIs
Publication statusPublished - dec. 2010

Fingerprint

Daunorubicin
Peptides
Cells
Cell-Penetrating Peptides
Cell Line
Macrophages
HL-60 Cells
Cytostatic Agents
Cytotoxicity
Pharmaceutical Preparations
Antineoplastic Agents
Bone
Leukemia
Adhesion
Water
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

Daunomycin-polypeptide conjugates with antitumor activity. / Szabó, Rita; Bánóczi, Z.; Mező, G.; Láng, Orsolya; Kőhidai, L.; Hudecz, F.

In: BBA - Biomembranes, Vol. 1798, No. 12, 12.2010, p. 2209-2216.

Research output: Article

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AU - Hudecz, F.

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