Cytotoxic T lymphocyte (CTL) responses human cytomegalovirus pp65, IE1- Exon4, gB, pp150, and pp28 in healthy individuals: Reevaluation of prevalence of IE1-specific CTLs

Zsofia Gyulai, Valeria Endresz, Katalin Burian, Steve Pincus, Joseph Toldy, William I. Cox, Claude Meric, Stanley Plotkin, Eva Gönczöl, Klara Berencsi

Research output: Article

114 Citations (Scopus)

Abstract

The prevalence of human cytomegalovirus (HCMV) pp65-, pp150-, IE1-exon4- , gB- and pp28-specific cytotoxic T lymphocyte (CTL) responses was compared among 34 healthy individuals, grouped by neutralizing antibody titers. Moderately and highly seropositive donors showed predominantly pp65- and IE1- exon4-specific CTL responses (92% and 76% of the donors, respectively), with similar precursor frequencies in the 2 donors tested. In addition, highly seropositive and a few moderately seropositive donors showed CTL responses to gB and pp150 (33% and 30% of the donors, respectively). No individual recognized pp28 as a target in the CTL assay. Phenotypic analysis revealed a mixed effector population of CD4+ and CD8+ (1 donor) or only CD8+ cells for pp65-specific effectors (2 donors). IE1-exon4 and pp150-specific effectors were CD8+ (2 donors and 1 donor, respectively), whereas gB- specific CTLs were CD4+ (1 donor). These data may help to design a cellular immunity-based vaccine effective against HCMV diseases.

Original languageEnglish
Pages (from-to)1537-1546
Number of pages10
JournalJournal of Infectious Diseases
Volume181
Issue number5
DOIs
Publication statusPublished - jún. 28 2000

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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