Cytochemistry of tubuloreticular structures in lymphocytes from patients with systemic lupus erythematosus and in cultured human lymphoid cells. Comparison to a paramyxovirus

Z. Schaff, D. W. Barry, P. M. Grimley

Research output: Article

74 Citations (Scopus)

Abstract

Tubuloreticular structures (TRS) which occur in the blood lymphocytes of patients with autoimmune disease and which can be induced in human lymphoid cell cultures by treatment with 5 bromodeoxyrudine were investigated cytochemically. In samples fixed with glutaraldehyde or osmium tetroxide and embedded in epoxy resin, the TRS appeared as networks of 20 to 30 nm. diameter membranous tubules within the endoplasmic reticulum. An electron dense material associated with the tubules was highlighted after embedding in glycol methacrylate or after fixation with potassium permanganate. The glycol methacrylate embedded material resisted digestion by pancreatic ribonuclease or deoxyribonuclease and stained intensely with the Rambourg method for glycoprotein. Although the constituent tubules of TRS are of 'virus like' proportions, there was no evidence for a relationship to similarly sized cores of a known paramyxovirus. In glycol methacrylate sections of chicken embryo cells infected by Newcastle disease virus, cytoplasmic inclusions of the paramyxovirus nucleocapsids were digested by the pancreatic ribonuclease treatment. The findings suggested that TRS consist principally of phospholipid and acidic glycoprotein.

Original languageEnglish
Pages (from-to)577-586
Number of pages10
JournalLaboratory Investigation
Volume29
Issue number6
Publication statusPublished - 1973

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Histocytochemistry
Systemic Lupus Erythematosus
Pancreatic Ribonuclease
Lymphocytes
Glycoproteins
Potassium Permanganate
Osmium Tetroxide
Epoxy Resins
Nucleocapsid
Newcastle disease virus
Viral Structures
Deoxyribonucleases
Inclusion Bodies
Glutaral
Endoplasmic Reticulum
Autoimmune Diseases
Digestion
Chickens
Phospholipids
Embryonic Structures

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

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title = "Cytochemistry of tubuloreticular structures in lymphocytes from patients with systemic lupus erythematosus and in cultured human lymphoid cells. Comparison to a paramyxovirus",
abstract = "Tubuloreticular structures (TRS) which occur in the blood lymphocytes of patients with autoimmune disease and which can be induced in human lymphoid cell cultures by treatment with 5 bromodeoxyrudine were investigated cytochemically. In samples fixed with glutaraldehyde or osmium tetroxide and embedded in epoxy resin, the TRS appeared as networks of 20 to 30 nm. diameter membranous tubules within the endoplasmic reticulum. An electron dense material associated with the tubules was highlighted after embedding in glycol methacrylate or after fixation with potassium permanganate. The glycol methacrylate embedded material resisted digestion by pancreatic ribonuclease or deoxyribonuclease and stained intensely with the Rambourg method for glycoprotein. Although the constituent tubules of TRS are of 'virus like' proportions, there was no evidence for a relationship to similarly sized cores of a known paramyxovirus. In glycol methacrylate sections of chicken embryo cells infected by Newcastle disease virus, cytoplasmic inclusions of the paramyxovirus nucleocapsids were digested by the pancreatic ribonuclease treatment. The findings suggested that TRS consist principally of phospholipid and acidic glycoprotein.",
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AU - Schaff, Z.

AU - Barry, D. W.

AU - Grimley, P. M.

PY - 1973

Y1 - 1973

N2 - Tubuloreticular structures (TRS) which occur in the blood lymphocytes of patients with autoimmune disease and which can be induced in human lymphoid cell cultures by treatment with 5 bromodeoxyrudine were investigated cytochemically. In samples fixed with glutaraldehyde or osmium tetroxide and embedded in epoxy resin, the TRS appeared as networks of 20 to 30 nm. diameter membranous tubules within the endoplasmic reticulum. An electron dense material associated with the tubules was highlighted after embedding in glycol methacrylate or after fixation with potassium permanganate. The glycol methacrylate embedded material resisted digestion by pancreatic ribonuclease or deoxyribonuclease and stained intensely with the Rambourg method for glycoprotein. Although the constituent tubules of TRS are of 'virus like' proportions, there was no evidence for a relationship to similarly sized cores of a known paramyxovirus. In glycol methacrylate sections of chicken embryo cells infected by Newcastle disease virus, cytoplasmic inclusions of the paramyxovirus nucleocapsids were digested by the pancreatic ribonuclease treatment. The findings suggested that TRS consist principally of phospholipid and acidic glycoprotein.

AB - Tubuloreticular structures (TRS) which occur in the blood lymphocytes of patients with autoimmune disease and which can be induced in human lymphoid cell cultures by treatment with 5 bromodeoxyrudine were investigated cytochemically. In samples fixed with glutaraldehyde or osmium tetroxide and embedded in epoxy resin, the TRS appeared as networks of 20 to 30 nm. diameter membranous tubules within the endoplasmic reticulum. An electron dense material associated with the tubules was highlighted after embedding in glycol methacrylate or after fixation with potassium permanganate. The glycol methacrylate embedded material resisted digestion by pancreatic ribonuclease or deoxyribonuclease and stained intensely with the Rambourg method for glycoprotein. Although the constituent tubules of TRS are of 'virus like' proportions, there was no evidence for a relationship to similarly sized cores of a known paramyxovirus. In glycol methacrylate sections of chicken embryo cells infected by Newcastle disease virus, cytoplasmic inclusions of the paramyxovirus nucleocapsids were digested by the pancreatic ribonuclease treatment. The findings suggested that TRS consist principally of phospholipid and acidic glycoprotein.

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