Correlations Between Prognosis and Regional Biomarker Profiles in Head and Neck Squamous Cell Carcinomas

Gabriella Szentkúti, Kornél Dános, Diána Brauswetter, Gergő Kiszner, T. Krenács, László Csákó, G. Répássy, László Tamás

Research output: Article

19 Citations (Scopus)

Abstract

Head and neck squamous cell carcinomas (HNSCC) show diverse clinicopathological features and are mostly linked with poor outcome. In this study, we tested if the expression of tumor growth, cell cycle and basement membrane anchorage related biomarkers allow prognostic and clinicopathological stratification of HNSCC. Archived HNSCC samples from 226 patients included into tissue microarrays (TMA) were tested using immunohistochemistry. Histopathological evaluation and the analysis of immunostaining for EGFR, Ki67, p53, p16ink4 and Collagen XVII proteins were carried out in digital whole slides. Statistical evaluation was carried out using Pearson’s Chi-square test and Kaplan-Meier survival analysis. In the tested cohort, hypopharyngeal cancers had the least favorable, and glottic cancers had the most favorable prognosis. High Ki67 positive tumor cell fractions were associated with significantly worse prognosis and elevated rate of lymph node metastasis. Both Ki67 and EGFR expression correlated significantly with the tumor localization. Ki67 index was the highest in the hypopharyngeal region and it proved to be the lowest in the glottic region. EGFR expression was the highest in the oral cavity and the lowest in the glottic region. The survival rate of patients with p16ink4-negative cancer was significantly lower than of those with p16ink4-positive disease. A significant inverse correlation was found between histological grade and the prognosis of HNSCC. Our data support that elevated Ki67 positive proliferating cell fractions contribute to the unfavorable prognosis of hypopharyngeal cancers, while glottic cancers have the most favorable prognosis because of the lowest Ki67 expression rate.

Original languageEnglish
Pages (from-to)643-650
Number of pages8
JournalPathology and Oncology Research
Volume21
Issue number3
DOIs
Publication statusPublished - dec. 31 2014

Fingerprint

Biomarkers
Hypopharyngeal Neoplasms
Tongue Neoplasms
Tongue
Neoplasms
Kaplan-Meier Estimate
Chi-Square Distribution
Survival Analysis
Basement Membrane
Mouth
Cell Cycle
Survival Rate
Lymph Nodes
Immunohistochemistry
Cell Membrane
Carcinoma, squamous cell of head and neck
Neoplasm Metastasis
Growth
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Correlations Between Prognosis and Regional Biomarker Profiles in Head and Neck Squamous Cell Carcinomas. / Szentkúti, Gabriella; Dános, Kornél; Brauswetter, Diána; Kiszner, Gergő; Krenács, T.; Csákó, László; Répássy, G.; Tamás, László.

In: Pathology and Oncology Research, Vol. 21, No. 3, 31.12.2014, p. 643-650.

Research output: Article

Szentkúti, Gabriella ; Dános, Kornél ; Brauswetter, Diána ; Kiszner, Gergő ; Krenács, T. ; Csákó, László ; Répássy, G. ; Tamás, László. / Correlations Between Prognosis and Regional Biomarker Profiles in Head and Neck Squamous Cell Carcinomas. In: Pathology and Oncology Research. 2014 ; Vol. 21, No. 3. pp. 643-650.
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AU - Kiszner, Gergő

AU - Krenács, T.

AU - Csákó, László

AU - Répássy, G.

AU - Tamás, László

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AB - Head and neck squamous cell carcinomas (HNSCC) show diverse clinicopathological features and are mostly linked with poor outcome. In this study, we tested if the expression of tumor growth, cell cycle and basement membrane anchorage related biomarkers allow prognostic and clinicopathological stratification of HNSCC. Archived HNSCC samples from 226 patients included into tissue microarrays (TMA) were tested using immunohistochemistry. Histopathological evaluation and the analysis of immunostaining for EGFR, Ki67, p53, p16ink4 and Collagen XVII proteins were carried out in digital whole slides. Statistical evaluation was carried out using Pearson’s Chi-square test and Kaplan-Meier survival analysis. In the tested cohort, hypopharyngeal cancers had the least favorable, and glottic cancers had the most favorable prognosis. High Ki67 positive tumor cell fractions were associated with significantly worse prognosis and elevated rate of lymph node metastasis. Both Ki67 and EGFR expression correlated significantly with the tumor localization. Ki67 index was the highest in the hypopharyngeal region and it proved to be the lowest in the glottic region. EGFR expression was the highest in the oral cavity and the lowest in the glottic region. The survival rate of patients with p16ink4-negative cancer was significantly lower than of those with p16ink4-positive disease. A significant inverse correlation was found between histological grade and the prognosis of HNSCC. Our data support that elevated Ki67 positive proliferating cell fractions contribute to the unfavorable prognosis of hypopharyngeal cancers, while glottic cancers have the most favorable prognosis because of the lowest Ki67 expression rate.

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