Coronary metabolic adaptation restricted by endothelin in the dog heart

Research output: Article

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Abstract

Endothelin elicits long-lasting vasoconstriction in the coronary bed. This remarkable spastic response raises the question whether or not the metabolic adaptive mechanisms of the coronaries are activated under endothelin effect. The role of the compensatory mediators adenosine and inosine was investigated before and after intracoronary (ic.) administration of endothelin-1 (ET-1, 1.0 nmol) using 1-min reactive hyperemia (RH) tests on in situ dog hearts (n=15) with or without blocking the ATP-sensitive potassium (K+ATP) channels by glibenclamide (GLIB, 1.0 μmol min-1, ic.). The release of adenosine and inosine via the coronary sinus was measured by HPLC during the first minute of RH. Endothelin-1 reduced baseline coronary blood flow (CBF) and RH response (hyperemic excess flow (EF) control vs. ET-1: 81.7±13.6 vs. 43.4±10.9 ml, P<0.01), while it increased the net nucleoside release (adenosine, control vs. ET-1: 58.9±20.4 vs. 113.7±39.4 nmol, P<0.05; inosine: 242.1±81.8 vs. 786.9±190.8 nmol, P<0.05). GLIB treatment alone did not change baseline CBF but also reduced RH significantly and increased nucleoside release (EF control vs. GLIB: 72.1±11.7 vs. 31.9±5.5 ml, P<0.01; adenosine: 18.8±4.6 vs. 63.0±24.8 nmol, P<0.05; inosine: 113.0±.37.2 vs. 328.2±127.5 nmol, P<0.05). Endothelin-1 on GLIB-treated coronaries further diminished RH and increased nucleoside release (EF: 21.5±8.0 ml, P<0.05 vs. GLIB; adenosine: 75.3±28.1 nmol, NS; inosine: 801.9±196.6 nmol, P<0.05 vs. GLIB).

Original languageEnglish
Pages (from-to)35-46
Number of pages12
JournalActa physiologica Hungarica
Volume88
Issue number1
DOIs
Publication statusPublished - dec. 1 2001

ASJC Scopus subject areas

  • Physiology (medical)

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