Core oxidative stress response in Aspergillus nidulans

T. Emri, Vera Szarvas, Erzsébet Orosz, K. Antal, HeeSoo S. Park, Kap Hoon Han, Jae Hyuk Yu, I. Pócsi

Research output: Article

18 Citations (Scopus)

Abstract

Background: The b-Zip transcription factor AtfA plays a key role in regulating stress responses in the filamentous fungus Aspergillus nidulans. To identify the core regulons of AtfA, we examined genome-wide expression changes caused by various stresses in the presence/absence of AtfA using A. nidulans microarrays. We also intended to address the intriguing question regarding the existence of core environmental stress response in this important model eukaryote. Results: Examination of the genome wide expression changes caused by five different oxidative stress conditions in wild type and the atfA null mutant has identified a significant number of stereotypically regulated genes (Core Oxidative Stress Response genes). The deletion of atfA increased the oxidative stress sensitivity of A. nidulans and affected mRNA accumulation of several genes under both unstressed and stressed conditions. The numbers of genes under the AtfA control appear to be specific to a stress-type. We also found that both oxidative and salt stresses induced expression of some secondary metabolite gene clusters and the deletion of atfA enhanced the stress responsiveness of additional clusters. Moreover, certain clusters were down-regulated by the stresses tested. Conclusion: Our data suggest that the observed co-regulations were most likely consequences of the overlapping physiological effects of the stressors and not of the existence of a general environmental stress response. The function of AtfA in governing various stress responses is much smaller than anticipated and/or other regulators may play a redundant or overlapping role with AtfA. Both stress inducible and stress repressive regulations of secondary metabolism seem to be frequent features in A. nidulans.

Original languageEnglish
Article number478
JournalBMC Genomics
Volume16
Issue number1
DOIs
Publication statusPublished - jún. 27 2015

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Aspergillus nidulans
Oxidative Stress
Genes
Genome
Secondary Metabolism
Regulon
Gene Deletion
Multigene Family
Eukaryota
Fungi
Transcription Factors
Salts
Messenger RNA

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

Core oxidative stress response in Aspergillus nidulans. / Emri, T.; Szarvas, Vera; Orosz, Erzsébet; Antal, K.; Park, HeeSoo S.; Han, Kap Hoon; Yu, Jae Hyuk; Pócsi, I.

In: BMC Genomics, Vol. 16, No. 1, 478, 27.06.2015.

Research output: Article

Emri, T. ; Szarvas, Vera ; Orosz, Erzsébet ; Antal, K. ; Park, HeeSoo S. ; Han, Kap Hoon ; Yu, Jae Hyuk ; Pócsi, I. / Core oxidative stress response in Aspergillus nidulans. In: BMC Genomics. 2015 ; Vol. 16, No. 1.
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abstract = "Background: The b-Zip transcription factor AtfA plays a key role in regulating stress responses in the filamentous fungus Aspergillus nidulans. To identify the core regulons of AtfA, we examined genome-wide expression changes caused by various stresses in the presence/absence of AtfA using A. nidulans microarrays. We also intended to address the intriguing question regarding the existence of core environmental stress response in this important model eukaryote. Results: Examination of the genome wide expression changes caused by five different oxidative stress conditions in wild type and the atfA null mutant has identified a significant number of stereotypically regulated genes (Core Oxidative Stress Response genes). The deletion of atfA increased the oxidative stress sensitivity of A. nidulans and affected mRNA accumulation of several genes under both unstressed and stressed conditions. The numbers of genes under the AtfA control appear to be specific to a stress-type. We also found that both oxidative and salt stresses induced expression of some secondary metabolite gene clusters and the deletion of atfA enhanced the stress responsiveness of additional clusters. Moreover, certain clusters were down-regulated by the stresses tested. Conclusion: Our data suggest that the observed co-regulations were most likely consequences of the overlapping physiological effects of the stressors and not of the existence of a general environmental stress response. The function of AtfA in governing various stress responses is much smaller than anticipated and/or other regulators may play a redundant or overlapping role with AtfA. Both stress inducible and stress repressive regulations of secondary metabolism seem to be frequent features in A. nidulans.",
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AU - Pócsi, I.

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