Copy number gain of PIK3CA and MET is associated with poor prognosis in head and neck squamous cell carcinoma

Diána Brauswetter, Kornél Dános, Bianka Gurbi, Éva Fruzsina Félegyházi, Ede Birtalan, Nóra Meggyesházi, T. Krenács, László Tamás, I. Peták

Research output: Article

12 Citations (Scopus)

Abstract

The incidence of head and neck squamous cell carcinomas is still growing, and the long-term prognosis of advanced disease remains poor. Only a fraction of head and neck cancers are sensitive to the EGFR-inhibitor cetuximab, which is the only registered targeted therapy available today. In several cancers, gene copy number alterations of MET and PIK3CA have been found to be prognostic and predictive for therapy response. The aim of this study was to systematically analyze in head and neck cancers the pathological characteristics and prognostic significance of copy number changes of MET and PIK3CA genes. MET and PIK3CA copy numbers were analyzed by fluorescence in situ hybridization in tumor samples of 152 patients. Expression of EGFR, p16, and Ki67 was studied by immunohistochemistry. High polysomy of PIK3CA (chromosome 3) was found in 20 % of cases and amplification in 4.5 %. Regarding MET, 35 % of cases showed low or high polysomy of the gene (chromosome 7), while no intra-chromosomal amplification of MET was detected. PIK3CA copy number gain (high polysomy or amplification) was significantly associated with shorter disease-specific survival, larger tumor volume, and lower p16 expression. MET copy number gain (low or high polysomy) in tumors was significantly associated with shorter disease-specific survival and lower level of EGFR. PIK3CA and MET may play an important role in oncogenesis of certain specific subtypes of head and neck cancer. There is an urgent need for the development of novel targeted therapies against these tumors associated with poor prognosis.

Original languageEnglish
Pages (from-to)579-587
Number of pages9
JournalVirchows Archiv
Volume468
Issue number5
DOIs
Publication statusPublished - máj. 1 2016

    Fingerprint

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Molecular Biology

Cite this

Brauswetter, D., Dános, K., Gurbi, B., Félegyházi, É. F., Birtalan, E., Meggyesházi, N., Krenács, T., Tamás, L., & Peták, I. (2016). Copy number gain of PIK3CA and MET is associated with poor prognosis in head and neck squamous cell carcinoma. Virchows Archiv, 468(5), 579-587. https://doi.org/10.1007/s00428-016-1905-1