In this work, we describe the controlled syntheses and detailed size, morphology, and structural characterization of gold/nanohybrid materials using lysozyme (LYZ) biopolymer. The LYZ is used to reduce directly the Au3+ ions without application of any other reducing agent and also stabilize the Au nanoparticles (NPs) and Au nanoclusters (NCs) in aqueous dispersions. The syntheses were carried out with varying metal ion/LYZ ratios at pH = 12 at 37 ± 0.1 °C. It was found that plasmonic Au NPs (d = 10–17 nm) with a compact structure or Au NCs (d <2 nm) with remarkable fluorescent features were formed depending on the applied Au/LYZ ratios. The conformational change of the LYZ, as an effect of Au/LYZ ratio, obtained from infrared (IR) measurements was compared with the small-angle X-ray scattering (SAXS) results. Fluorescence quenching studies have also been performed in order to get detailed information on the mechanism of Au/LYZ interaction.
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Polymers and Plastics
- Materials Chemistry
- Colloid and Surface Chemistry