Construction of a basic genetic map for alfalfa using RFLP, RAPD, isozyme and morphological markers

G. Kiss, Gyula Csanádi, Katalin Kálmán, P. Kaló, L. Ökrész

Research output: Article

110 Citations (Scopus)

Abstract

The genetic map for alfalfa presented here has eight linkage groups representing the haploid chromosome set of the Medicago species. The genetic map was constructed by ordering the linkage values of 89 RFLP, RAPD, isozyme and morphological markers collected from a segregating population of 138 individuals. The segregating population is self-mated progeny of an F1 hybrid plant deriving from a cross between the diploid (2 n=2 x=16) yellow-flowered Medicago sativa ssp. quasifalcata and the diploid (2 n=2 x=16) blue-flowered M. sativa ssp. coerulea. The inheritance of many traits displayed distorted segregation, indicating the presence of lethal loci in the heterozygotic parent plants. In spite of the lack of uniform segregation, linkage groups could be assigned and the order of the markers spanning > 659 centimorgans could be unambiguously determined. This value and the calculated haploid genome size for Medicago (1 n=1 x=1.0 x 109 bp) gives a ratio of <1500 kb per centimorgan.

Original languageEnglish
Pages (from-to)129-137
Number of pages9
JournalMGG Molecular & General Genetics
Volume238
Issue number1-2
DOIs
Publication statusPublished - ápr. 1993

Fingerprint

Medicago sativa
Restriction Fragment Length Polymorphisms
Isoenzymes
Medicago
Haploidy
Diploidy
Genome Size
Population
Chromosomes

ASJC Scopus subject areas

  • Genetics

Cite this

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title = "Construction of a basic genetic map for alfalfa using RFLP, RAPD, isozyme and morphological markers",
abstract = "The genetic map for alfalfa presented here has eight linkage groups representing the haploid chromosome set of the Medicago species. The genetic map was constructed by ordering the linkage values of 89 RFLP, RAPD, isozyme and morphological markers collected from a segregating population of 138 individuals. The segregating population is self-mated progeny of an F1 hybrid plant deriving from a cross between the diploid (2 n=2 x=16) yellow-flowered Medicago sativa ssp. quasifalcata and the diploid (2 n=2 x=16) blue-flowered M. sativa ssp. coerulea. The inheritance of many traits displayed distorted segregation, indicating the presence of lethal loci in the heterozygotic parent plants. In spite of the lack of uniform segregation, linkage groups could be assigned and the order of the markers spanning > 659 centimorgans could be unambiguously determined. This value and the calculated haploid genome size for Medicago (1 n=1 x=1.0 x 109 bp) gives a ratio of <1500 kb per centimorgan.",
keywords = "Linkage map, Medicago, Nodulins, RFLP mapping, Symbiotic nitrogen fixation",
author = "G. Kiss and Gyula Csan{\'a}di and Katalin K{\'a}lm{\'a}n and P. Kal{\'o} and L. {\"O}kr{\'e}sz",
year = "1993",
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T1 - Construction of a basic genetic map for alfalfa using RFLP, RAPD, isozyme and morphological markers

AU - Kiss, G.

AU - Csanádi, Gyula

AU - Kálmán, Katalin

AU - Kaló, P.

AU - Ökrész, L.

PY - 1993/4

Y1 - 1993/4

N2 - The genetic map for alfalfa presented here has eight linkage groups representing the haploid chromosome set of the Medicago species. The genetic map was constructed by ordering the linkage values of 89 RFLP, RAPD, isozyme and morphological markers collected from a segregating population of 138 individuals. The segregating population is self-mated progeny of an F1 hybrid plant deriving from a cross between the diploid (2 n=2 x=16) yellow-flowered Medicago sativa ssp. quasifalcata and the diploid (2 n=2 x=16) blue-flowered M. sativa ssp. coerulea. The inheritance of many traits displayed distorted segregation, indicating the presence of lethal loci in the heterozygotic parent plants. In spite of the lack of uniform segregation, linkage groups could be assigned and the order of the markers spanning > 659 centimorgans could be unambiguously determined. This value and the calculated haploid genome size for Medicago (1 n=1 x=1.0 x 109 bp) gives a ratio of <1500 kb per centimorgan.

AB - The genetic map for alfalfa presented here has eight linkage groups representing the haploid chromosome set of the Medicago species. The genetic map was constructed by ordering the linkage values of 89 RFLP, RAPD, isozyme and morphological markers collected from a segregating population of 138 individuals. The segregating population is self-mated progeny of an F1 hybrid plant deriving from a cross between the diploid (2 n=2 x=16) yellow-flowered Medicago sativa ssp. quasifalcata and the diploid (2 n=2 x=16) blue-flowered M. sativa ssp. coerulea. The inheritance of many traits displayed distorted segregation, indicating the presence of lethal loci in the heterozygotic parent plants. In spite of the lack of uniform segregation, linkage groups could be assigned and the order of the markers spanning > 659 centimorgans could be unambiguously determined. This value and the calculated haploid genome size for Medicago (1 n=1 x=1.0 x 109 bp) gives a ratio of <1500 kb per centimorgan.

KW - Linkage map

KW - Medicago

KW - Nodulins

KW - RFLP mapping

KW - Symbiotic nitrogen fixation

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