Consequence of O2·- generation during a bacterially induced hypersensitive reaction in tobacco

deterioration of membrane lipids

A. Adám, T. Farkas, G. Somlyai, M. Hevesi, Z. Király

Research output: Article

180 Citations (Scopus)

Abstract

Changes in the lipid composition of host cell membranes were characterized during the hypersensitive reaction (HR) induced by Pseudomonas syringae pv. syringae in plants of Nicotiana tabacum cv. Burley 21. Before the appearance of symptoms of the HR, and before the decrease in the amounts of phospholipids and galactolipids, increased O2·- generation and lipid peroxidation were detected as well as reductions in the degree of unsaturation of polar acyl lipids and in the free sterol pool. Inoculation with a Tn5 transposon mutant of Pseudomonas syringae pv. phaseolicola, which had lost the ability to induce an HR did not result in these changes. Application of different types of free radical scavengers, such as ascorbic acid, reduced glutathione, α-tocopherol-acetate and bovine serum albumin, delayed the development of the HR. These results are consistent with the hypothesis that membrane deterioration during bacterially induced HR is caused by various forms of activated oxygen.

Original languageEnglish
Pages (from-to)13-26
Number of pages14
JournalPhysiological and Molecular Plant Pathology
Volume34
Issue number1
DOIs
Publication statusPublished - 1989

Fingerprint

Pseudomonas syringae
hypersensitive response
Membrane Lipids
Tobacco
tobacco
Galactolipids
deterioration
Lipids
Free Radical Scavengers
alpha-Tocopherol
Sterols
lipids
Bovine Serum Albumin
Lipid Peroxidation
Ascorbic Acid
Glutathione
Phospholipids
Cell Membrane
Oxygen
Pseudomonas syringae pv. phaseolicola

ASJC Scopus subject areas

  • Plant Science

Cite this

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title = "Consequence of O2·- generation during a bacterially induced hypersensitive reaction in tobacco: deterioration of membrane lipids",
abstract = "Changes in the lipid composition of host cell membranes were characterized during the hypersensitive reaction (HR) induced by Pseudomonas syringae pv. syringae in plants of Nicotiana tabacum cv. Burley 21. Before the appearance of symptoms of the HR, and before the decrease in the amounts of phospholipids and galactolipids, increased O2·- generation and lipid peroxidation were detected as well as reductions in the degree of unsaturation of polar acyl lipids and in the free sterol pool. Inoculation with a Tn5 transposon mutant of Pseudomonas syringae pv. phaseolicola, which had lost the ability to induce an HR did not result in these changes. Application of different types of free radical scavengers, such as ascorbic acid, reduced glutathione, α-tocopherol-acetate and bovine serum albumin, delayed the development of the HR. These results are consistent with the hypothesis that membrane deterioration during bacterially induced HR is caused by various forms of activated oxygen.",
author = "A. Ad{\'a}m and T. Farkas and G. Somlyai and M. Hevesi and Z. Kir{\'a}ly",
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T1 - Consequence of O2·- generation during a bacterially induced hypersensitive reaction in tobacco

T2 - deterioration of membrane lipids

AU - Adám, A.

AU - Farkas, T.

AU - Somlyai, G.

AU - Hevesi, M.

AU - Király, Z.

PY - 1989

Y1 - 1989

N2 - Changes in the lipid composition of host cell membranes were characterized during the hypersensitive reaction (HR) induced by Pseudomonas syringae pv. syringae in plants of Nicotiana tabacum cv. Burley 21. Before the appearance of symptoms of the HR, and before the decrease in the amounts of phospholipids and galactolipids, increased O2·- generation and lipid peroxidation were detected as well as reductions in the degree of unsaturation of polar acyl lipids and in the free sterol pool. Inoculation with a Tn5 transposon mutant of Pseudomonas syringae pv. phaseolicola, which had lost the ability to induce an HR did not result in these changes. Application of different types of free radical scavengers, such as ascorbic acid, reduced glutathione, α-tocopherol-acetate and bovine serum albumin, delayed the development of the HR. These results are consistent with the hypothesis that membrane deterioration during bacterially induced HR is caused by various forms of activated oxygen.

AB - Changes in the lipid composition of host cell membranes were characterized during the hypersensitive reaction (HR) induced by Pseudomonas syringae pv. syringae in plants of Nicotiana tabacum cv. Burley 21. Before the appearance of symptoms of the HR, and before the decrease in the amounts of phospholipids and galactolipids, increased O2·- generation and lipid peroxidation were detected as well as reductions in the degree of unsaturation of polar acyl lipids and in the free sterol pool. Inoculation with a Tn5 transposon mutant of Pseudomonas syringae pv. phaseolicola, which had lost the ability to induce an HR did not result in these changes. Application of different types of free radical scavengers, such as ascorbic acid, reduced glutathione, α-tocopherol-acetate and bovine serum albumin, delayed the development of the HR. These results are consistent with the hypothesis that membrane deterioration during bacterially induced HR is caused by various forms of activated oxygen.

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