Connexin 43 differentially regulates epileptiform activity in models of convulsive and non-convulsive epilepsies

Renáta Vincze, Márton Péter, Zsolt Szabó, J. Kardos, László Héja, Z. Kovács

Research output: Article

Abstract

The influence of astrocytic cell networks on neuronal network activity is an emerging issue in epilepsy. Among the various mechanisms by which astrocytes modulate neuronal function, synchronization of astrocytes via gap junction channels is widely considered to be a crucial mechanism in epileptic conditions, contributing to the synchronization of the neuronal cell networks, possibly inducing recurrent epileptiform activity. Here, we explored whether modulation of astrocytic gap junctions could alter epileptic seizures in different types of epilepsy. Opening of gap junctions by trimethylamine intensifies seizure-like events (SLEs) in the low-[Mg2+] in vitro model of temporal lobe epilepsy, while alleviates seizures in the in vivo WAG/Rij rat model of absence epilepsy. In contrast, application of the gap junction blocker carbenoxolone prevents the appearance of SLEs in the low-[Mg2+] epilepsy model, but aggravates seizures in non-convulsive absence epilepsy, in vivo. Pharmacological dissection of neuronal vs. astrocytic connexins shows that astrocytic Cx43 contribute to seizure formation to a significantly higher extent than neuronal Cx36. We conclude that astrocytic gap junctions are key players in the formation of epileptiform activity and we provide a scheme for the different mode of action in the convulsive and non-convulsive epilepsy types.

Original languageEnglish
Article number173
JournalFrontiers in Cellular Neuroscience
Volume13
DOIs
Publication statusPublished - ápr. 12 2019

Fingerprint

Connexin 43
Gap Junctions
Epilepsy
Seizures
Absence Epilepsy
Astrocytes
Carbenoxolone
Connexins
Temporal Lobe Epilepsy
Dissection
Pharmacology

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Connexin 43 differentially regulates epileptiform activity in models of convulsive and non-convulsive epilepsies. / Vincze, Renáta; Péter, Márton; Szabó, Zsolt; Kardos, J.; Héja, László; Kovács, Z.

In: Frontiers in Cellular Neuroscience, Vol. 13, 173, 12.04.2019.

Research output: Article

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AU - Héja, László

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