Conditional circadian regulation of phytochrome A gene expression

Anthony Hall, L. Kozma-Bognár, Réka Tóth, Ferenc Nagy, Andrew J. Millar

Research output: Article

53 Citations (Scopus)

Abstract

The phytochrome photoreceptors and the circadian clock control many of the same developmental processes, in all organs and throughout the growth of Arabidopsis plants. Phytochrome A (phyA) provides light input signals to entrain the circadian clock. The clock is known to rhythmically regulate its light input pathway, so we tested rhythmic regulation of phyA, using transgenic plants carrying a PHYA promoter fusion to the luciferase reporter (PHYA:LUC). We provide the first images of LUC activity with subcellular resolution in intact tissue. PHYA transcription and the accumulation of all three PHYA mRNAs were indeed clock controlled. PHYA is expressed throughout the seedling, so we tested whether circadian rhythms were observed in all PHYA-expressing organs and whether the rhythms were autonomously controlled by each organ. In contrast to our previous results using other clock controlled genes, the rhythmic pattern of PHYA expression varied markedly among isolated organs and between isolated organs and intact plants. High-amplitude rhythms were maintained for many days in isolated leaves in darkness, whereas the leaves of intact plants rapidly lost rhythmicity. Wounding the leaves of intact plants had no effect. The rhythmic pattern of PHYA expression is not organ autonomous but depends upon the physical continuity or isolation of the rhythmic tissues, consistent with the presence of a transmitted signal that controls the overt expression of circadian rhythms without necessarily affecting the underlying clock. A circadian system might be present in most, if not all, plant cells, but its effect on intracellular rhythms can be controlled by supracellular signaling.

Original languageEnglish
Pages (from-to)1808-1818
Number of pages11
JournalPlant Physiology
Volume127
Issue number4
DOIs
Publication statusPublished - jan. 1 2001

Fingerprint

Phytochrome A
Circadian Clocks
Plant Leaves
phytochrome
Circadian Rhythm
circadian rhythm
Phytochrome
Gene Expression
Light
gene expression
Genetically Modified Plants
Darkness
Plant Cells
Periodicity
Luciferases
Seedlings
Arabidopsis
Messenger RNA
Growth
leaves

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Conditional circadian regulation of phytochrome A gene expression. / Hall, Anthony; Kozma-Bognár, L.; Tóth, Réka; Nagy, Ferenc; Millar, Andrew J.

In: Plant Physiology, Vol. 127, No. 4, 01.01.2001, p. 1808-1818.

Research output: Article

Hall, Anthony ; Kozma-Bognár, L. ; Tóth, Réka ; Nagy, Ferenc ; Millar, Andrew J. / Conditional circadian regulation of phytochrome A gene expression. In: Plant Physiology. 2001 ; Vol. 127, No. 4. pp. 1808-1818.
@article{423ab113949f4704a5c93f8626dc1e82,
title = "Conditional circadian regulation of phytochrome A gene expression",
abstract = "The phytochrome photoreceptors and the circadian clock control many of the same developmental processes, in all organs and throughout the growth of Arabidopsis plants. Phytochrome A (phyA) provides light input signals to entrain the circadian clock. The clock is known to rhythmically regulate its light input pathway, so we tested rhythmic regulation of phyA, using transgenic plants carrying a PHYA promoter fusion to the luciferase reporter (PHYA:LUC). We provide the first images of LUC activity with subcellular resolution in intact tissue. PHYA transcription and the accumulation of all three PHYA mRNAs were indeed clock controlled. PHYA is expressed throughout the seedling, so we tested whether circadian rhythms were observed in all PHYA-expressing organs and whether the rhythms were autonomously controlled by each organ. In contrast to our previous results using other clock controlled genes, the rhythmic pattern of PHYA expression varied markedly among isolated organs and between isolated organs and intact plants. High-amplitude rhythms were maintained for many days in isolated leaves in darkness, whereas the leaves of intact plants rapidly lost rhythmicity. Wounding the leaves of intact plants had no effect. The rhythmic pattern of PHYA expression is not organ autonomous but depends upon the physical continuity or isolation of the rhythmic tissues, consistent with the presence of a transmitted signal that controls the overt expression of circadian rhythms without necessarily affecting the underlying clock. A circadian system might be present in most, if not all, plant cells, but its effect on intracellular rhythms can be controlled by supracellular signaling.",
author = "Anthony Hall and L. Kozma-Bogn{\'a}r and R{\'e}ka T{\'o}th and Ferenc Nagy and Millar, {Andrew J.}",
year = "2001",
month = "1",
day = "1",
doi = "10.1104/pp.010294",
language = "English",
volume = "127",
pages = "1808--1818",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "4",

}

TY - JOUR

T1 - Conditional circadian regulation of phytochrome A gene expression

AU - Hall, Anthony

AU - Kozma-Bognár, L.

AU - Tóth, Réka

AU - Nagy, Ferenc

AU - Millar, Andrew J.

PY - 2001/1/1

Y1 - 2001/1/1

N2 - The phytochrome photoreceptors and the circadian clock control many of the same developmental processes, in all organs and throughout the growth of Arabidopsis plants. Phytochrome A (phyA) provides light input signals to entrain the circadian clock. The clock is known to rhythmically regulate its light input pathway, so we tested rhythmic regulation of phyA, using transgenic plants carrying a PHYA promoter fusion to the luciferase reporter (PHYA:LUC). We provide the first images of LUC activity with subcellular resolution in intact tissue. PHYA transcription and the accumulation of all three PHYA mRNAs were indeed clock controlled. PHYA is expressed throughout the seedling, so we tested whether circadian rhythms were observed in all PHYA-expressing organs and whether the rhythms were autonomously controlled by each organ. In contrast to our previous results using other clock controlled genes, the rhythmic pattern of PHYA expression varied markedly among isolated organs and between isolated organs and intact plants. High-amplitude rhythms were maintained for many days in isolated leaves in darkness, whereas the leaves of intact plants rapidly lost rhythmicity. Wounding the leaves of intact plants had no effect. The rhythmic pattern of PHYA expression is not organ autonomous but depends upon the physical continuity or isolation of the rhythmic tissues, consistent with the presence of a transmitted signal that controls the overt expression of circadian rhythms without necessarily affecting the underlying clock. A circadian system might be present in most, if not all, plant cells, but its effect on intracellular rhythms can be controlled by supracellular signaling.

AB - The phytochrome photoreceptors and the circadian clock control many of the same developmental processes, in all organs and throughout the growth of Arabidopsis plants. Phytochrome A (phyA) provides light input signals to entrain the circadian clock. The clock is known to rhythmically regulate its light input pathway, so we tested rhythmic regulation of phyA, using transgenic plants carrying a PHYA promoter fusion to the luciferase reporter (PHYA:LUC). We provide the first images of LUC activity with subcellular resolution in intact tissue. PHYA transcription and the accumulation of all three PHYA mRNAs were indeed clock controlled. PHYA is expressed throughout the seedling, so we tested whether circadian rhythms were observed in all PHYA-expressing organs and whether the rhythms were autonomously controlled by each organ. In contrast to our previous results using other clock controlled genes, the rhythmic pattern of PHYA expression varied markedly among isolated organs and between isolated organs and intact plants. High-amplitude rhythms were maintained for many days in isolated leaves in darkness, whereas the leaves of intact plants rapidly lost rhythmicity. Wounding the leaves of intact plants had no effect. The rhythmic pattern of PHYA expression is not organ autonomous but depends upon the physical continuity or isolation of the rhythmic tissues, consistent with the presence of a transmitted signal that controls the overt expression of circadian rhythms without necessarily affecting the underlying clock. A circadian system might be present in most, if not all, plant cells, but its effect on intracellular rhythms can be controlled by supracellular signaling.

UR - http://www.scopus.com/inward/record.url?scp=85047682363&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047682363&partnerID=8YFLogxK

U2 - 10.1104/pp.010294

DO - 10.1104/pp.010294

M3 - Article

C2 - 11743124

AN - SCOPUS:85047682363

VL - 127

SP - 1808

EP - 1818

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 4

ER -