Comparison of ethylene glycol and propylene glycol for the vitrification of immature porcine oocytes

Tamás Somfai, Michiko Nakai, Fuminori Tanihara, Junko Noguchi, Hiroyuki Kaneko, Naomi Kashiwazaki, István Egerszegi, Takashi Nagai, Kazuhiro Kikuchi

Research output: Article

23 Citations (Scopus)

Abstract

Our aim was to optimize a cryoprotectant treatment for vitrification of immature porcine cumulus-oocyte complexes (COCs). Immature COCs were vitrified either in 35% ethylene glycol (EG), 35% propylene glycol (PG) or a combination of 17.5% EG and 17.5% PG. After warming, the COCs were in vitro matured (IVM), and surviving oocytes were in vitro fertilized (IVF) and cultured. The mean survival rate of vitrified oocytes in 35% PG (73.9%) was higher (P<0.05) than that in 35% EG (27.8%). Oocyte maturation rates did not differ among vitrified and non-vitrified control groups. Blastocyst formation in the vitrified EG group (10.8%) was higher (P<0.05) than that in the vitrified PG group (2.0%) but was lower than that in the control group (25.0%). Treatment of oocytes with 35% of each cryoprotectant without vitrification revealed a higher toxicity of PG on subsequent blastocyst development compared with EG. The combination of EG and PG resulted in 42.6% survival after vitrification. The maturation and fertilization rates of the surviving oocytes were similar in the vitrified, control and toxicity control (TC; treated with EG+PG combination without cooling) groups. Blastocyst development in the vitrified group was lower (P<0.05) than that in the control and TC groups, which in turn had similar development rates (10.7%, 18.1% and 23.3%, respectively). In conclusion, 35% PG enabled a higher oocyte survival rate after vitrification compared with 35% EG. However, PG was greatly toxic to oocytes. The combination of 17.5% EG and 17.5% PG yielded reasonable survival rates without toxic effects on embryo development.

Original languageEnglish
Pages (from-to)378-384
Number of pages7
JournalJournal of Reproduction and Development
Volume59
Issue number4
DOIs
Publication statusPublished - 2013

ASJC Scopus subject areas

  • Animal Science and Zoology

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    Somfai, T., Nakai, M., Tanihara, F., Noguchi, J., Kaneko, H., Kashiwazaki, N., Egerszegi, I., Nagai, T., & Kikuchi, K. (2013). Comparison of ethylene glycol and propylene glycol for the vitrification of immature porcine oocytes. Journal of Reproduction and Development, 59(4), 378-384. https://doi.org/10.1262/jrd.2013-015