Common NOD2/CARD15 variants are not associated with susceptibility or the clinicopathologic characteristics of sporadic colorectal cancer in Hungarian patients

P. Lakatos, E. Hitre, F. Szalay, Kerstin Zinober, P. Fuszek, L. Lakatos, S. Fischer, J. Osztovits, Orsolya Gemela, G. Verès, J. Papp, Peter Ferenci

Research output: Article

20 Citations (Scopus)

Abstract

Background: Epidemiological observations suggest that cancer arises from chronically inflamed tissues. Inflammatory bowel disease (IBD) is a typical example as patients with longstanding IBD are at an increased risk for developing colorectal cancer (CRC) and mutations of the NOD2/ CARD15 gene increase the risk for Crohn's disease (CD). Recently, NOD2/ CARD15 has been associated with a risk for CRC in some studies, which stemmed from ethnically diverse populations. Our aim was to identify common NOD2/CARD15 mutations in Hungarian patients with sporadic CRC. Methods: A total of 194 sporadic CRC patients (m/f: 108/86, age at diagnosis of CRC: 63.2 ± 9.1 years old) and 200 healthy subjects were included. DNA was screened for SNP8, SNP12 and SNP13 NOD2/CARD15 mutations by denaturing-HPLC and confirmed by direct sequencing. Results: NOD2/CARD15 mutations were found in 28 patients (14.4%) and in 23 controls (11.5%, p = NS). Allele frequencies for SNP8/R702W (1.8% vs. 1.5%) SNP12/G908R (1.8% vs. 1.8%) and SNP13/3020insC (3.6% vs. 2.5%) were also not statistically different between patients and controls. The clinicopathologic characteristics of CRC patients with or without NOD2/CARD15 mutations were not significantly different. Conclusion: Our results suggest that common NOD2/CARD15 mutations alone do not contribute to CRC risk in the Hungarian population.

Original languageEnglish
Article number54
JournalBMC Cancer
Volume7
DOIs
Publication statusPublished - márc. 27 2007

Fingerprint

Colorectal Neoplasms
Mutation
Inflammatory Bowel Diseases
Gene Frequency
Crohn Disease
Population
Healthy Volunteers
High Pressure Liquid Chromatography
DNA
Genes
Neoplasms

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

@article{74af19e13245483fb572d1237d6c7e4c,
title = "Common NOD2/CARD15 variants are not associated with susceptibility or the clinicopathologic characteristics of sporadic colorectal cancer in Hungarian patients",
abstract = "Background: Epidemiological observations suggest that cancer arises from chronically inflamed tissues. Inflammatory bowel disease (IBD) is a typical example as patients with longstanding IBD are at an increased risk for developing colorectal cancer (CRC) and mutations of the NOD2/ CARD15 gene increase the risk for Crohn's disease (CD). Recently, NOD2/ CARD15 has been associated with a risk for CRC in some studies, which stemmed from ethnically diverse populations. Our aim was to identify common NOD2/CARD15 mutations in Hungarian patients with sporadic CRC. Methods: A total of 194 sporadic CRC patients (m/f: 108/86, age at diagnosis of CRC: 63.2 ± 9.1 years old) and 200 healthy subjects were included. DNA was screened for SNP8, SNP12 and SNP13 NOD2/CARD15 mutations by denaturing-HPLC and confirmed by direct sequencing. Results: NOD2/CARD15 mutations were found in 28 patients (14.4{\%}) and in 23 controls (11.5{\%}, p = NS). Allele frequencies for SNP8/R702W (1.8{\%} vs. 1.5{\%}) SNP12/G908R (1.8{\%} vs. 1.8{\%}) and SNP13/3020insC (3.6{\%} vs. 2.5{\%}) were also not statistically different between patients and controls. The clinicopathologic characteristics of CRC patients with or without NOD2/CARD15 mutations were not significantly different. Conclusion: Our results suggest that common NOD2/CARD15 mutations alone do not contribute to CRC risk in the Hungarian population.",
author = "P. Lakatos and E. Hitre and F. Szalay and Kerstin Zinober and P. Fuszek and L. Lakatos and S. Fischer and J. Osztovits and Orsolya Gemela and G. Ver{\`e}s and J. Papp and Peter Ferenci",
year = "2007",
month = "3",
day = "27",
doi = "10.1186/1471-2407-7-54",
language = "English",
volume = "7",
journal = "BMC Cancer",
issn = "1471-2407",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Common NOD2/CARD15 variants are not associated with susceptibility or the clinicopathologic characteristics of sporadic colorectal cancer in Hungarian patients

AU - Lakatos, P.

AU - Hitre, E.

AU - Szalay, F.

AU - Zinober, Kerstin

AU - Fuszek, P.

AU - Lakatos, L.

AU - Fischer, S.

AU - Osztovits, J.

AU - Gemela, Orsolya

AU - Verès, G.

AU - Papp, J.

AU - Ferenci, Peter

PY - 2007/3/27

Y1 - 2007/3/27

N2 - Background: Epidemiological observations suggest that cancer arises from chronically inflamed tissues. Inflammatory bowel disease (IBD) is a typical example as patients with longstanding IBD are at an increased risk for developing colorectal cancer (CRC) and mutations of the NOD2/ CARD15 gene increase the risk for Crohn's disease (CD). Recently, NOD2/ CARD15 has been associated with a risk for CRC in some studies, which stemmed from ethnically diverse populations. Our aim was to identify common NOD2/CARD15 mutations in Hungarian patients with sporadic CRC. Methods: A total of 194 sporadic CRC patients (m/f: 108/86, age at diagnosis of CRC: 63.2 ± 9.1 years old) and 200 healthy subjects were included. DNA was screened for SNP8, SNP12 and SNP13 NOD2/CARD15 mutations by denaturing-HPLC and confirmed by direct sequencing. Results: NOD2/CARD15 mutations were found in 28 patients (14.4%) and in 23 controls (11.5%, p = NS). Allele frequencies for SNP8/R702W (1.8% vs. 1.5%) SNP12/G908R (1.8% vs. 1.8%) and SNP13/3020insC (3.6% vs. 2.5%) were also not statistically different between patients and controls. The clinicopathologic characteristics of CRC patients with or without NOD2/CARD15 mutations were not significantly different. Conclusion: Our results suggest that common NOD2/CARD15 mutations alone do not contribute to CRC risk in the Hungarian population.

AB - Background: Epidemiological observations suggest that cancer arises from chronically inflamed tissues. Inflammatory bowel disease (IBD) is a typical example as patients with longstanding IBD are at an increased risk for developing colorectal cancer (CRC) and mutations of the NOD2/ CARD15 gene increase the risk for Crohn's disease (CD). Recently, NOD2/ CARD15 has been associated with a risk for CRC in some studies, which stemmed from ethnically diverse populations. Our aim was to identify common NOD2/CARD15 mutations in Hungarian patients with sporadic CRC. Methods: A total of 194 sporadic CRC patients (m/f: 108/86, age at diagnosis of CRC: 63.2 ± 9.1 years old) and 200 healthy subjects were included. DNA was screened for SNP8, SNP12 and SNP13 NOD2/CARD15 mutations by denaturing-HPLC and confirmed by direct sequencing. Results: NOD2/CARD15 mutations were found in 28 patients (14.4%) and in 23 controls (11.5%, p = NS). Allele frequencies for SNP8/R702W (1.8% vs. 1.5%) SNP12/G908R (1.8% vs. 1.8%) and SNP13/3020insC (3.6% vs. 2.5%) were also not statistically different between patients and controls. The clinicopathologic characteristics of CRC patients with or without NOD2/CARD15 mutations were not significantly different. Conclusion: Our results suggest that common NOD2/CARD15 mutations alone do not contribute to CRC risk in the Hungarian population.

UR - http://www.scopus.com/inward/record.url?scp=34147185351&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34147185351&partnerID=8YFLogxK

U2 - 10.1186/1471-2407-7-54

DO - 10.1186/1471-2407-7-54

M3 - Article

C2 - 17389035

AN - SCOPUS:34147185351

VL - 7

JO - BMC Cancer

JF - BMC Cancer

SN - 1471-2407

M1 - 54

ER -