Collision induced dissociation study of the major components of silymarin

Ákos Kuki, Bernadett Biri, Lajos Nagy, György Deák, József Kalmár, Attila Mándi, Miklós Nagy, Miklós Zsuga, Sándor Kéki

Research output: Article

5 Citations (Scopus)

Abstract

The fragmentation properties of the major silymarin constituents, including silydianin, silychristin A and B, silybin A and B and isosilybin A and B were studied by energy-dependent collision-induced dissociation (CID) technique. The survival yield (SY) method was applied to compare the fragmentation behavior of the various silymarin components. MS/MS spectra were recorded at collision energies which correspond to about 50% survival yield (CE 50) and about 1% survival yield (CE 01). The laboratory frame collision energy dependence of the fragmentation was studied on the basis of the breakdown curves, i.e., the relative intensities of fragment ions versus collision energy. The fragmentation of silybins and isosilybins is distinct in spite of the minimal difference between their structures. This phenomenon, which is the basis of their analytical identification, is explained in details for the first time, on the basis of the energy-dependent MS/MS experiments and the conformational analysis.

Original languageEnglish
Pages (from-to)46-54
Number of pages9
JournalInternational Journal of Mass Spectrometry
Volume315
DOIs
Publication statusPublished - ápr. 1 2012

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ASJC Scopus subject areas

  • Instrumentation
  • Condensed Matter Physics
  • Spectroscopy
  • Physical and Theoretical Chemistry

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