Coagglutination test for serotyping Pasteurella haemolytica

L. Fodor, Z. Pénzes, J. Varga

Research output: Article

12 Citations (Scopus)

Abstract

A coagglutination test was described for simple, fast, and reliable detection of Pasteurella haemolytica type-specific antigens in lung lesions even in the absence of viable P. haemolytica. The coagglutinating reagents were prepared by coating protein A-producing Staphylococcus aureus cells with hyperimmune sera raised against P. haemolytica type strains. Bacterial suspensions, saline extracts, and boiled saline extracts of the bacteria were used as antigens. Homologous reactions with all types of antigens were precise. Some cross-reactions were similar to those obtained by the indirect hemagglutination test, and some additional one-way cross-reactions were identified. The coagglutination test was used for serotyping 65 P. haemolytica field strains and for the detection of P. haemolytica type- specific antigens in the lung specimens of 62 calves and 78 sheep. Ninety- four percent of the field strains could be serotyped by the coagglutination test. P. haemolytica type-specific antigens were detected in the lung specimens of 3 calves and 5 sheep that had succumbed to naturally occurring P. haemolytica pneumonia and in the lungs of 20 calves experimentally infected with P. haemolytica A1. The coagglutination test detected type- specific antigens in 36% of the lung specimens of slaughtered field sheep but not in the lungs of slaughtered field cattle with small chronic lung lesions. No reaction occurred in the case of nonpneumonic calves and sheep or when pneumonic lesions were caused by other bacteria. No P. haemolytica strains could be isolated from lung samples that were coagglutination test negative. This test is recommended as an additional method for fast and reliable serotyping of P. haemolytica.

Original languageEnglish
Pages (from-to)393-397
Number of pages5
JournalJournal of Clinical Microbiology
Volume34
Issue number2
Publication statusPublished - 1996

Fingerprint

Mannheimia haemolytica
Serotyping
Lung
Antigens
Sheep
Cross Reactions
Bacteria
Hemagglutination Tests
Staphylococcal Protein A
Staphylococcus aureus
Pneumonia
Suspensions

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Coagglutination test for serotyping Pasteurella haemolytica. / Fodor, L.; Pénzes, Z.; Varga, J.

In: Journal of Clinical Microbiology, Vol. 34, No. 2, 1996, p. 393-397.

Research output: Article

@article{b28a101b63cf4afbb08674f45cbfe54e,
title = "Coagglutination test for serotyping Pasteurella haemolytica",
abstract = "A coagglutination test was described for simple, fast, and reliable detection of Pasteurella haemolytica type-specific antigens in lung lesions even in the absence of viable P. haemolytica. The coagglutinating reagents were prepared by coating protein A-producing Staphylococcus aureus cells with hyperimmune sera raised against P. haemolytica type strains. Bacterial suspensions, saline extracts, and boiled saline extracts of the bacteria were used as antigens. Homologous reactions with all types of antigens were precise. Some cross-reactions were similar to those obtained by the indirect hemagglutination test, and some additional one-way cross-reactions were identified. The coagglutination test was used for serotyping 65 P. haemolytica field strains and for the detection of P. haemolytica type- specific antigens in the lung specimens of 62 calves and 78 sheep. Ninety- four percent of the field strains could be serotyped by the coagglutination test. P. haemolytica type-specific antigens were detected in the lung specimens of 3 calves and 5 sheep that had succumbed to naturally occurring P. haemolytica pneumonia and in the lungs of 20 calves experimentally infected with P. haemolytica A1. The coagglutination test detected type- specific antigens in 36{\%} of the lung specimens of slaughtered field sheep but not in the lungs of slaughtered field cattle with small chronic lung lesions. No reaction occurred in the case of nonpneumonic calves and sheep or when pneumonic lesions were caused by other bacteria. No P. haemolytica strains could be isolated from lung samples that were coagglutination test negative. This test is recommended as an additional method for fast and reliable serotyping of P. haemolytica.",
author = "L. Fodor and Z. P{\'e}nzes and J. Varga",
year = "1996",
language = "English",
volume = "34",
pages = "393--397",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "2",

}

TY - JOUR

T1 - Coagglutination test for serotyping Pasteurella haemolytica

AU - Fodor, L.

AU - Pénzes, Z.

AU - Varga, J.

PY - 1996

Y1 - 1996

N2 - A coagglutination test was described for simple, fast, and reliable detection of Pasteurella haemolytica type-specific antigens in lung lesions even in the absence of viable P. haemolytica. The coagglutinating reagents were prepared by coating protein A-producing Staphylococcus aureus cells with hyperimmune sera raised against P. haemolytica type strains. Bacterial suspensions, saline extracts, and boiled saline extracts of the bacteria were used as antigens. Homologous reactions with all types of antigens were precise. Some cross-reactions were similar to those obtained by the indirect hemagglutination test, and some additional one-way cross-reactions were identified. The coagglutination test was used for serotyping 65 P. haemolytica field strains and for the detection of P. haemolytica type- specific antigens in the lung specimens of 62 calves and 78 sheep. Ninety- four percent of the field strains could be serotyped by the coagglutination test. P. haemolytica type-specific antigens were detected in the lung specimens of 3 calves and 5 sheep that had succumbed to naturally occurring P. haemolytica pneumonia and in the lungs of 20 calves experimentally infected with P. haemolytica A1. The coagglutination test detected type- specific antigens in 36% of the lung specimens of slaughtered field sheep but not in the lungs of slaughtered field cattle with small chronic lung lesions. No reaction occurred in the case of nonpneumonic calves and sheep or when pneumonic lesions were caused by other bacteria. No P. haemolytica strains could be isolated from lung samples that were coagglutination test negative. This test is recommended as an additional method for fast and reliable serotyping of P. haemolytica.

AB - A coagglutination test was described for simple, fast, and reliable detection of Pasteurella haemolytica type-specific antigens in lung lesions even in the absence of viable P. haemolytica. The coagglutinating reagents were prepared by coating protein A-producing Staphylococcus aureus cells with hyperimmune sera raised against P. haemolytica type strains. Bacterial suspensions, saline extracts, and boiled saline extracts of the bacteria were used as antigens. Homologous reactions with all types of antigens were precise. Some cross-reactions were similar to those obtained by the indirect hemagglutination test, and some additional one-way cross-reactions were identified. The coagglutination test was used for serotyping 65 P. haemolytica field strains and for the detection of P. haemolytica type- specific antigens in the lung specimens of 62 calves and 78 sheep. Ninety- four percent of the field strains could be serotyped by the coagglutination test. P. haemolytica type-specific antigens were detected in the lung specimens of 3 calves and 5 sheep that had succumbed to naturally occurring P. haemolytica pneumonia and in the lungs of 20 calves experimentally infected with P. haemolytica A1. The coagglutination test detected type- specific antigens in 36% of the lung specimens of slaughtered field sheep but not in the lungs of slaughtered field cattle with small chronic lung lesions. No reaction occurred in the case of nonpneumonic calves and sheep or when pneumonic lesions were caused by other bacteria. No P. haemolytica strains could be isolated from lung samples that were coagglutination test negative. This test is recommended as an additional method for fast and reliable serotyping of P. haemolytica.

UR - http://www.scopus.com/inward/record.url?scp=0030050573&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030050573&partnerID=8YFLogxK

M3 - Article

C2 - 8789022

AN - SCOPUS:0030050573

VL - 34

SP - 393

EP - 397

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 2

ER -