Cloning and sequences of the structural (hupSLC) and accessory (hupDHI) genes for hydrogenase biosynthesis in Thiocapsa roseopersicina

Annette Colbeau, K. Kovács, Jacqueline Chabert, Paulette M. Vignais

Research output: Article

37 Citations (Scopus)

Abstract

The first molecular biology study on the purple sulfur photosynthetic bacterium Thiocapsa roseopersicina is reported, namely, the construction of cosmid libraries and isolation of a hydrogenase gene cluster by hybridization with hydrogenase structural genes from the purple non-sulfur bacterium, Rhodobacter capsulatus. The sequenced gene cluster contains six open reading frames, the products of which show significant degrees of identity (from 40 to 78%) with hydrogenase gene products necessary for biosynthesis of the group-I of [NiFe]hydrogenases. The structural hupSLC genes encode the small and large hydrogenase subunits and a hydrophobic protein shown to accept electrons from hydrogenase in R. capsulatus. They are followed downstream by three genes, hupDHI, which are similar to hydrogenase accessory genes found in other bacteria.

Original languageEnglish
Pages (from-to)25-31
Number of pages7
JournalGene
Volume140
Issue number1
DOIs
Publication statusPublished - 1994

Fingerprint

Thiocapsa roseopersicina
Hydrogenase
Organism Cloning
Chromatiaceae
Rhodobacter capsulatus
Genes
Multigene Family
Cosmids
Open Reading Frames
Molecular Biology
Electrons
Bacteria

ASJC Scopus subject areas

  • Genetics

Cite this

Cloning and sequences of the structural (hupSLC) and accessory (hupDHI) genes for hydrogenase biosynthesis in Thiocapsa roseopersicina. / Colbeau, Annette; Kovács, K.; Chabert, Jacqueline; Vignais, Paulette M.

In: Gene, Vol. 140, No. 1, 1994, p. 25-31.

Research output: Article

Colbeau, Annette ; Kovács, K. ; Chabert, Jacqueline ; Vignais, Paulette M. / Cloning and sequences of the structural (hupSLC) and accessory (hupDHI) genes for hydrogenase biosynthesis in Thiocapsa roseopersicina. In: Gene. 1994 ; Vol. 140, No. 1. pp. 25-31.
@article{3bbe57fff1c64523bd57d7f5d334ce43,
title = "Cloning and sequences of the structural (hupSLC) and accessory (hupDHI) genes for hydrogenase biosynthesis in Thiocapsa roseopersicina",
abstract = "The first molecular biology study on the purple sulfur photosynthetic bacterium Thiocapsa roseopersicina is reported, namely, the construction of cosmid libraries and isolation of a hydrogenase gene cluster by hybridization with hydrogenase structural genes from the purple non-sulfur bacterium, Rhodobacter capsulatus. The sequenced gene cluster contains six open reading frames, the products of which show significant degrees of identity (from 40 to 78{\%}) with hydrogenase gene products necessary for biosynthesis of the group-I of [NiFe]hydrogenases. The structural hupSLC genes encode the small and large hydrogenase subunits and a hydrophobic protein shown to accept electrons from hydrogenase in R. capsulatus. They are followed downstream by three genes, hupDHI, which are similar to hydrogenase accessory genes found in other bacteria.",
keywords = "cosmid library, hydrogen metabolism, membrane metalloenzyme, Photosynthetic bacteria, purple sulfur bacteria, [NiFe] hydrogenase",
author = "Annette Colbeau and K. Kov{\'a}cs and Jacqueline Chabert and Vignais, {Paulette M.}",
year = "1994",
doi = "10.1016/0378-1119(94)90726-9",
language = "English",
volume = "140",
pages = "25--31",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Cloning and sequences of the structural (hupSLC) and accessory (hupDHI) genes for hydrogenase biosynthesis in Thiocapsa roseopersicina

AU - Colbeau, Annette

AU - Kovács, K.

AU - Chabert, Jacqueline

AU - Vignais, Paulette M.

PY - 1994

Y1 - 1994

N2 - The first molecular biology study on the purple sulfur photosynthetic bacterium Thiocapsa roseopersicina is reported, namely, the construction of cosmid libraries and isolation of a hydrogenase gene cluster by hybridization with hydrogenase structural genes from the purple non-sulfur bacterium, Rhodobacter capsulatus. The sequenced gene cluster contains six open reading frames, the products of which show significant degrees of identity (from 40 to 78%) with hydrogenase gene products necessary for biosynthesis of the group-I of [NiFe]hydrogenases. The structural hupSLC genes encode the small and large hydrogenase subunits and a hydrophobic protein shown to accept electrons from hydrogenase in R. capsulatus. They are followed downstream by three genes, hupDHI, which are similar to hydrogenase accessory genes found in other bacteria.

AB - The first molecular biology study on the purple sulfur photosynthetic bacterium Thiocapsa roseopersicina is reported, namely, the construction of cosmid libraries and isolation of a hydrogenase gene cluster by hybridization with hydrogenase structural genes from the purple non-sulfur bacterium, Rhodobacter capsulatus. The sequenced gene cluster contains six open reading frames, the products of which show significant degrees of identity (from 40 to 78%) with hydrogenase gene products necessary for biosynthesis of the group-I of [NiFe]hydrogenases. The structural hupSLC genes encode the small and large hydrogenase subunits and a hydrophobic protein shown to accept electrons from hydrogenase in R. capsulatus. They are followed downstream by three genes, hupDHI, which are similar to hydrogenase accessory genes found in other bacteria.

KW - cosmid library

KW - hydrogen metabolism

KW - membrane metalloenzyme

KW - Photosynthetic bacteria

KW - purple sulfur bacteria

KW - [NiFe] hydrogenase

UR - http://www.scopus.com/inward/record.url?scp=0028167135&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028167135&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(94)90726-9

DO - 10.1016/0378-1119(94)90726-9

M3 - Article

VL - 140

SP - 25

EP - 31

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1

ER -