Cleavage of pre-mRNA sequences by ribonucleases bound to nuclear RNP particles of rat liver

J. Molnár, G. Bajszár, I. Marczinovits, G. Szabó

Research output: Article

3 Citations (Scopus)

Abstract

The 30S nuclear RNP particles from rat liver have been shown to split the double-stranded- (ds) and single-stranded (ss) sequences of nuclear pre-mRNA. Experiments performed in vitro have demonstrated that 1) a 5′-exonuclease and an endonuclease specific for double-stranded pre-mRNA sequences exist in the 30S pre-mRNP particles; 2) in dsRNA monophosphorylated 5′-termini arose in the course of incubation with 30S RNP and most of the products remained double-stranded. The analysis of terminal pNp nucleotides revealed a relatively high ratio of pPyp in the cleaved dsRNA, whereas the nucleosides in 5′-terminal pNp of ssRNA showed nearly random distribution. Our results provide a possible explanation for the appearance of pNp termini during the processing of nuclear pre-mRNA of mammalian cells.

Original languageEnglish
Pages (from-to)157-161
Number of pages5
JournalMolecular Biology Reports
Volume4
Issue number3
DOIs
Publication statusPublished - szept. 1 1978

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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