Chiral separation of α-amino acids by ligand-exchange capillary electrophoresis using N-(2-hydroxy-octyl)-L-4-hydroxyproline as a selector

Ákos Végvári, Martin G. Schmid, F. Kilár, Gerald Gübitz

Research output: Article

54 Citations (Scopus)

Abstract

The direct chiral resolution of underivatized α-amino acids by capillary zone electrophoresis (CZE) based on the principle of ligand exchange is described. An N-(2-hydroxyoctyl)-L-4-hydroxyproline/Cu(II) complex was used as a chiral selector. Besides amino acids containing aromatic residues, the basic amino acid histidine was resolved. Baseline separations were obtained for all amino acids investigated. The influence of selector concentration, electrolyte composition and pH on the resolution was investigated. It was found that there is a correlation between pI of the amino acids and the optimal pH.

Original languageEnglish
Pages (from-to)2109-2112
Number of pages4
JournalElectrophoresis
Volume19
Issue number12
DOIs
Publication statusPublished - szept. 1998

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Ion exchange
Ligands
Amino Acids
Basic Amino Acids
Aromatic Amino Acids
Histidine
Electrolytes
Hydroxyproline
Electrophoresis
N-(2-hydroxyoctyl)-4-hydroxyproline
Chemical analysis

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Chiral separation of α-amino acids by ligand-exchange capillary electrophoresis using N-(2-hydroxy-octyl)-L-4-hydroxyproline as a selector. / Végvári, Ákos; Schmid, Martin G.; Kilár, F.; Gübitz, Gerald.

In: Electrophoresis, Vol. 19, No. 12, 09.1998, p. 2109-2112.

Research output: Article

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