The beneficial effect of trans-resveratrol (RESV) on health is well documented. Our aim was to study the putative preventive effect of RESV on the cytotoxicity of frequently used herbicides (alachlor, acetochlor). Estrogen receptor positive (ER+) MCF-7 human mammary carcinoma, HepG2 (ER+) human hepatocellular carcinoma and VERO estrogen receptor negative (ER-) non-transformed monkey fibroblast cell lines were treated with alachlor and acetochlor (2-500 μg/ml) as toxic agents, and RESV (10 μM) as preventive agent. The MTT dye reduction assay was performed to test cytotoxicity, and flow cytometry to test cell proliferation and apoptosis. RESV is not cytotoxic in the concentration range of 1-100 μM on neither cell lines examined after 24 h, but cytotoxic on Vero and MCF-7 cells at 100 μM after 48 h, and on all three cell lines after 72 h. On both ER+ cell lines a stimulation of viability occurs in the low concentration range (0.5-12.5 μM) as detected by the MTT assay. Cell cycle analysis of the culture shows a significant increase of S-phase cells at low concentrations of RESV (10-50 μM) and a decrease in the 100-200 μM concentration range. The ratio of apoptotic cells significantly increases after the administration of 50 μM RESV, depending on the incubation time. The cytotoxicity of 20-65 μg/ml alachlor and 10-65 μg/ml acetochlor was significantly decreased by the addition of 10 μM RESV in Vero ER- cells whereas no significant change was detected on ER+ cell lines MCF-7 and HepG2. These results show that RESV protects non-transformed ER- cells, but has no such effect on ER+ tumor cells.
|Number of pages||8|
|Journal||International Journal of Hygiene and Environmental Health|
|Publication status||Published - máj. 13 2005|
ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health