Characterization of rabbit κ-casein cDNA: Control of κ-casein gene expression in vivo and in vitro

Z. Bösze, E. Devinoy, C. Puissant, M. L. Fontaine, L. M. Houdebine

Research output: Article

13 Citations (Scopus)

Abstract

The rabbit κ-casein cDNA was cloned and sequenced. One of the isolated clones included almost the entire 5' end, while another clone corresponded to the 3' end of the cDNA. No polyadenylation site was found and therefore this clone did not harbour the complete cDNA. The amino acid sequence of a full-length protein was deduced from the nucleotide sequence obtained for this partial cDNA. It revealed the presence of a chymosin cleavage site and five potential phosphorylation sites. Rabbit κ-casein was compared with those already described in other species. The rabbit sequence is closer to the ovine than to the mouse sequence. This result supports the idea that Lagomorpha are not closer to Rodentia than to Artiodactyla. The cDNA described above was used to study κ-casein gene expression in the rabbit mammary gland. This expression was induced primarily by prolactin in mammary gland organoids and was similar to α(s1)-casein gene expression in vivo. The κ-casein gene present in the casein gene locus is thus subject to the same regulation as the α(s1)-casein gene, although it has evolved from a fibrinogen gene.

Original languageEnglish
Pages (from-to)9-17
Number of pages9
JournalJournal of Molecular Endocrinology
Volume11
Issue number1
Publication statusPublished - 1993

Fingerprint

Caseins
Complementary DNA
Rabbits
Gene Expression
Clone Cells
Human Mammary Glands
Genes
Artiodactyla
Lagomorpha
Chymosin
Organoids
Polyadenylation
In Vitro Techniques
Prolactin
Fibrinogen
Amino Acid Sequence
Rodentia
Sheep
Phosphorylation
Proteins

ASJC Scopus subject areas

  • Endocrinology

Cite this

Characterization of rabbit κ-casein cDNA : Control of κ-casein gene expression in vivo and in vitro. / Bösze, Z.; Devinoy, E.; Puissant, C.; Fontaine, M. L.; Houdebine, L. M.

In: Journal of Molecular Endocrinology, Vol. 11, No. 1, 1993, p. 9-17.

Research output: Article

Bösze, Z. ; Devinoy, E. ; Puissant, C. ; Fontaine, M. L. ; Houdebine, L. M. / Characterization of rabbit κ-casein cDNA : Control of κ-casein gene expression in vivo and in vitro. In: Journal of Molecular Endocrinology. 1993 ; Vol. 11, No. 1. pp. 9-17.
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