Characterization of photomorphogenic responses and signaling cascades controlled by phytochrome-A expressed in different tissues

Daniel Kirchenbauer, András Viczián, E. Ádám, Z. Hegedűs, Cornelia Klose, Michael Leppert, Andreas Hiltbrunner, Stefan Kircher, Eberhard Schäfer, Ferenc Nagy

Research output: Article

10 Citations (Scopus)

Abstract

The photoreceptor phytochrome A acts as a light-dependent molecular switch and regulates responses initiated by very low fluences of light (VLFR) and high fluences (HIR) of far-red light. PhyA is expressed ubiquitously, but how phyA signaling is orchestrated to regulate photomorphogenesis is poorly understood. To address this issue, we generated transgenic Arabidopsis thaliana phyA-201 mutant lines expressing the biologically active phyA-YFP photoreceptor in different tissues, and analyzed the expression of several reporter genes, including ProHY5:HY5-GFP and Pro35S:CFP-PIF1, and various FR-HIR-dependent physiological responses. We show that phyA action in one tissue is critical and sufficient to regulate flowering time and root growth; control of cotyledon and hypocotyl growth requires simultaneous phyA activity in different tissues; and changes detected in the expression of reporters are not restricted to phyA-containing cells. We conclude that FR-HIR-controlled morphogenesis in Arabidopsis is mediated partly by tissue-specific and partly by intercellular signaling initiated by phyA. Intercellular signaling is critical for many FR-HIR induced responses, yet it appears that phyA modulates the abundance and activity of key regulatory transcription factors in a tissue-autonomous fashion.

Original languageEnglish
Pages (from-to)584-598
Number of pages15
JournalThe New phytologist
Volume211
Issue number2
DOIs
Publication statusPublished - júl. 1 2016

Fingerprint

Phytochrome A
phytochrome
photoreceptors
Arabidopsis
Light
Hypocotyl
photomorphogenesis
Cotyledon
far-red light
Growth
Morphogenesis
Reporter Genes
reporter genes
physiological response
hypocotyls
morphogenesis
cotyledons
root growth
Transcription Factors
Arabidopsis thaliana

Keywords

    ASJC Scopus subject areas

    • Physiology
    • Plant Science

    Cite this

    Characterization of photomorphogenic responses and signaling cascades controlled by phytochrome-A expressed in different tissues. / Kirchenbauer, Daniel; Viczián, András; Ádám, E.; Hegedűs, Z.; Klose, Cornelia; Leppert, Michael; Hiltbrunner, Andreas; Kircher, Stefan; Schäfer, Eberhard; Nagy, Ferenc.

    In: The New phytologist, Vol. 211, No. 2, 01.07.2016, p. 584-598.

    Research output: Article

    Kirchenbauer, D, Viczián, A, Ádám, E, Hegedűs, Z, Klose, C, Leppert, M, Hiltbrunner, A, Kircher, S, Schäfer, E & Nagy, F 2016, 'Characterization of photomorphogenic responses and signaling cascades controlled by phytochrome-A expressed in different tissues' The New phytologist, vol. 211, no. 2, pp. 584-598. https://doi.org/10.1111/nph.13941
    Kirchenbauer, Daniel ; Viczián, András ; Ádám, E. ; Hegedűs, Z. ; Klose, Cornelia ; Leppert, Michael ; Hiltbrunner, Andreas ; Kircher, Stefan ; Schäfer, Eberhard ; Nagy, Ferenc. / Characterization of photomorphogenic responses and signaling cascades controlled by phytochrome-A expressed in different tissues. In: The New phytologist. 2016 ; Vol. 211, No. 2. pp. 584-598.
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