Characterization of peroxidase secretion and subcellular organization of Phanerochaete chrysosporium INA-12 in the presence of various soybean phospholipid fractions

C. Capdevila, S. Moukha, M. Ghyczy-, J. Theilleux, B. Gelie, M. Delattre, G. Corrieu, M. Asther

Research output: Article

27 Citations (Scopus)

Abstract

Stimulation of lignin peroxidase production by exogenous phospholipids depends on the composition of the phospholipid fraction prepared by using the Nattermann process. The fraction composed mainly of negatively charged phospholipids (NAT 89) was the most efficient source for exoprotein secretion by Phanerochaete chrysosporium INA-12. The results of biochemical marker assays and ultrastructural morphology determination by electron microscopy were correlated. Activities of succinate dehydrogenase, a mitochondrial marker, and cytochrome c oxidoreductase, an endoplasmic reticulum (ER) marker, were increased 1.3- and 2.2-fold, respectively, in the presence of NAT 89. Electron microscopy observations suggested that the amount of mitochondria and ER in culture containing phospholipids was increased at the optimum day of lignin peroxidase production. Therefore, phospholipids enhanced energetic metabolism of strain INA-12 and markedly modified fungus physiology. Since ER is involved in enzyme synthesis, we suggest that its increased amount in mycelium cultured with NAT 89 is directly associated with the higher production of lignin peroxidase.

Original languageEnglish
Pages (from-to)3811-3816
Number of pages6
JournalApplied and Environmental Microbiology
Volume56
Issue number12
Publication statusPublished - 1990

Fingerprint

Phanerochaete
Phanerochaete chrysosporium
phospholipid
Soybeans
secretion
Peroxidase
soybean
lignin peroxidase
Phospholipids
phospholipids
peroxidase
soybeans
Endoplasmic Reticulum
endoplasmic reticulum
lignin
electron microscopy
Electron Microscopy
fungus physiology
Succinate Dehydrogenase
Mycelium

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Characterization of peroxidase secretion and subcellular organization of Phanerochaete chrysosporium INA-12 in the presence of various soybean phospholipid fractions. / Capdevila, C.; Moukha, S.; Ghyczy-, M.; Theilleux, J.; Gelie, B.; Delattre, M.; Corrieu, G.; Asther, M.

In: Applied and Environmental Microbiology, Vol. 56, No. 12, 1990, p. 3811-3816.

Research output: Article

Capdevila, C. ; Moukha, S. ; Ghyczy-, M. ; Theilleux, J. ; Gelie, B. ; Delattre, M. ; Corrieu, G. ; Asther, M. / Characterization of peroxidase secretion and subcellular organization of Phanerochaete chrysosporium INA-12 in the presence of various soybean phospholipid fractions. In: Applied and Environmental Microbiology. 1990 ; Vol. 56, No. 12. pp. 3811-3816.
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T1 - Characterization of peroxidase secretion and subcellular organization of Phanerochaete chrysosporium INA-12 in the presence of various soybean phospholipid fractions

AU - Capdevila, C.

AU - Moukha, S.

AU - Ghyczy-, M.

AU - Theilleux, J.

AU - Gelie, B.

AU - Delattre, M.

AU - Corrieu, G.

AU - Asther, M.

PY - 1990

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AB - Stimulation of lignin peroxidase production by exogenous phospholipids depends on the composition of the phospholipid fraction prepared by using the Nattermann process. The fraction composed mainly of negatively charged phospholipids (NAT 89) was the most efficient source for exoprotein secretion by Phanerochaete chrysosporium INA-12. The results of biochemical marker assays and ultrastructural morphology determination by electron microscopy were correlated. Activities of succinate dehydrogenase, a mitochondrial marker, and cytochrome c oxidoreductase, an endoplasmic reticulum (ER) marker, were increased 1.3- and 2.2-fold, respectively, in the presence of NAT 89. Electron microscopy observations suggested that the amount of mitochondria and ER in culture containing phospholipids was increased at the optimum day of lignin peroxidase production. Therefore, phospholipids enhanced energetic metabolism of strain INA-12 and markedly modified fungus physiology. Since ER is involved in enzyme synthesis, we suggest that its increased amount in mycelium cultured with NAT 89 is directly associated with the higher production of lignin peroxidase.

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