Characterization of human Spartan/C1orf124, an ubiquitin-PCNA interacting regulator of DNA damage tolerance

Szilvia Juhasz, David Balogh, Ildiko Hajdu, Peter Burkovics, Mark A. Villamil, Zhihao Zhuang, L. Haracska

Research output: Article

57 Citations (Scopus)

Abstract

Unrepaired DNA damage may arrest ongoing replication forks, potentially resulting in fork collapse, increased mutagenesis and genomic instability. Replication through DNA lesions depends on mono-and polyubiquitylation of proliferating cell nuclear antigen (PCNA), which enable translesion synthesis (TLS) and template switching, respectively. A proper replication fork rescue is ensured by the dynamic ubiquitylation and deubiquitylation of PCNA; however, as yet, little is known about its regulation. Here, we show that human Spartan/C1orf124 protein provides a higher cellular level of ubiquitylated-PCNA by which it regulates the choice of DNA damage tolerance pathways. We find that Spartan is recruited to sites of replication stress, a process that depends on its PCNA-and ubiquitin-interacting domains and the RAD18 PCNA ubiquitin ligase. Preferential association of Spartan with ubiquitin-modified PCNA protects against PCNA deubiquitylation by ubiquitin-specific protease 1 and facilitates the access of a TLS polymerase to the replication fork. In concert, depletion of Spartan leads to increased sensitivity to DNA damaging agents and causes elevated levels of sister chromatid exchanges. We propose that Spartan promotes genomic stability by regulating the choice of rescue of stalled replication fork, whose mechanism includes its interaction with ubiquitin-conjugated PCNA and protection against PCNA deubiquitylation.

Original languageEnglish
Pages (from-to)10795-10808
Number of pages14
JournalNucleic Acids Research
Volume40
Issue number21
DOIs
Publication statusPublished - nov. 2012

Fingerprint

Proliferating Cell Nuclear Antigen
Ubiquitin
DNA Damage
Genomic Instability
Ubiquitin-Specific Proteases
Sister Chromatid Exchange
Ubiquitination
Ligases
DNA Replication
Mutagenesis
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Characterization of human Spartan/C1orf124, an ubiquitin-PCNA interacting regulator of DNA damage tolerance. / Juhasz, Szilvia; Balogh, David; Hajdu, Ildiko; Burkovics, Peter; Villamil, Mark A.; Zhuang, Zhihao; Haracska, L.

In: Nucleic Acids Research, Vol. 40, No. 21, 11.2012, p. 10795-10808.

Research output: Article

Juhasz, Szilvia ; Balogh, David ; Hajdu, Ildiko ; Burkovics, Peter ; Villamil, Mark A. ; Zhuang, Zhihao ; Haracska, L. / Characterization of human Spartan/C1orf124, an ubiquitin-PCNA interacting regulator of DNA damage tolerance. In: Nucleic Acids Research. 2012 ; Vol. 40, No. 21. pp. 10795-10808.
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