Characterization of enhancing factor (EF) prepared from spleen of mice immunized with tetanus toxoid.

I. Barna-Vetró, R. Varró, E. Osz

Research output: Article

Abstract

In cultures of spleen cells from tetanus toxoid-primed mice a soluble, nonspecific material appeared, which enhanced antibody response in vivo. The active material was purified by gel filtration on Sephadex-G-150 and by affinity chromatography on Con-A lectin. According to the immunological and physiochemical investigation the active material does not contain carbohydrate and its molecular weight is in the 100 000 dalton range.

Original languageEnglish
Pages (from-to)143-148
Number of pages6
JournalAnnales Immunologiae Hungaricae
Volume19
Publication statusPublished - 1979

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Tetanus Toxoid
Affinity Chromatography
Lectins
Antibody Formation
Gel Chromatography
Spleen
Cell Culture Techniques
Molecular Weight
Carbohydrates
sephadex

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "Characterization of enhancing factor (EF) prepared from spleen of mice immunized with tetanus toxoid.",
abstract = "In cultures of spleen cells from tetanus toxoid-primed mice a soluble, nonspecific material appeared, which enhanced antibody response in vivo. The active material was purified by gel filtration on Sephadex-G-150 and by affinity chromatography on Con-A lectin. According to the immunological and physiochemical investigation the active material does not contain carbohydrate and its molecular weight is in the 100 000 dalton range.",
author = "I. Barna-Vetr{\'o} and R. Varr{\'o} and E. Osz",
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journal = "Annales Immunologiae Hungaricae",
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AU - Varró, R.

AU - Osz, E.

PY - 1979

Y1 - 1979

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AB - In cultures of spleen cells from tetanus toxoid-primed mice a soluble, nonspecific material appeared, which enhanced antibody response in vivo. The active material was purified by gel filtration on Sephadex-G-150 and by affinity chromatography on Con-A lectin. According to the immunological and physiochemical investigation the active material does not contain carbohydrate and its molecular weight is in the 100 000 dalton range.

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