Characterization of canine pericardial fluid endothelin-1 levels

István Szokodi, Ferenc Horkay, Pál Kiss, László Selmeci, Iván Horváth, Olli Vuolteenaho, Heikki Ruskoaho, Alexander Juhász-Nagy, Miklós Tóth

Research output: Article

10 Citations (Scopus)

Abstract

Recently, extremely high concentrations of endothelin-1 (ET-1) were detected in the pericardial fluid of patients undergoing cardiac surgery. This study was designed to characterize ET-1 levels in plasma and pericardial fluid of dogs. Plasma and pericardial fluid samples were extracted using SepPak C18 cartridges and ET-1 levels were measured by a specific radioimmunoassay. Pericardial fluid ET-1 levels (739 ± 81 pg/ml; n = 10) were significantly higher than respective plasma levels (22.4 ± 4.0 pg/ml; p < 0.05). The mean pericardial fluid: plasma ratio of ir-ET-1 concentration was 54.7 ± 16.8. In HPLC analysis, the total ET-1-like immunoreactivity of pericardial fluid co-eluted with ET-1 standard. In anesthetized dogs (n = 6), disappearance of [125I]ET-1 was sixfold slower in the pericardial space than in plasma. Our results show that high concentrations of ir-ET-1 can be found in canine pericardial fluid. Slow elimination of ET-1 from the pericardial fluid compartment may contribute to high peptide levels.

Original languageEnglish
Pages (from-to)S399-S400
JournalJournal of cardiovascular pharmacology
Volume31
Issue numberSUPPL. 1
DOIs
Publication statusPublished - jan. 1 1998

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

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