Characterisation of the nucleic acid binding features of the PRRSV 7ap and its ability to induce antinuclear antibodies

F. Olasz, B. Dénes, A. Bálint, Tibor Magyar, Sándor Belak, Z. Zádori

Research output: Article

2 Citations (Scopus)

Abstract

A short alternative open reading frame named ORF7a has recently been discovered within the nucleocapsid gene of the porcine reproductive and respiratory syndrome virus (PRRSV) genome. Proteins (7ap) translated from the ORF7a of two divergent strains -a type I and a type II -are able to completely reduce the motility of nucleic acids at relatively high molar charge ratios in gel retardation assays indicating strong dsDNA-and ssRNA-binding capability. Conserved RNAand DNA-binding properties suggest that nucleic acid binding is a functional property of the divergent 7aps, and not an arbitrary consequence of their net positive charge. Sera from Hu7ap-immunised pigs and mice did not react with Hu7ap or Hu7ap-GFP; however, antinuclear antibodies were detected in the sera of the immunised animals, suggesting an ability of Hu7ap to interact with or mimic autoantigenic macromolecules.

Original languageEnglish
Pages (from-to)124-134
Number of pages11
JournalActa Veterinaria Hungarica
Volume65
Issue number1
DOIs
Publication statusPublished - jan. 1 2017

Fingerprint

Porcine respiratory and reproductive syndrome virus
Porcine reproductive and respiratory syndrome virus
Antinuclear Antibodies
Nucleic Acids
nucleic acids
Nucleocapsid
nucleocapsid
antibodies
binding properties
Electrophoretic Mobility Shift Assay
Serum
Open Reading Frames
functional properties
open reading frames
Swine
gels
Genome
swine
genome
DNA

ASJC Scopus subject areas

  • veterinary(all)

Cite this

@article{99dd1364a7584865baf55291ee6a3b46,
title = "Characterisation of the nucleic acid binding features of the PRRSV 7ap and its ability to induce antinuclear antibodies",
abstract = "A short alternative open reading frame named ORF7a has recently been discovered within the nucleocapsid gene of the porcine reproductive and respiratory syndrome virus (PRRSV) genome. Proteins (7ap) translated from the ORF7a of two divergent strains -a type I and a type II -are able to completely reduce the motility of nucleic acids at relatively high molar charge ratios in gel retardation assays indicating strong dsDNA-and ssRNA-binding capability. Conserved RNAand DNA-binding properties suggest that nucleic acid binding is a functional property of the divergent 7aps, and not an arbitrary consequence of their net positive charge. Sera from Hu7ap-immunised pigs and mice did not react with Hu7ap or Hu7ap-GFP; however, antinuclear antibodies were detected in the sera of the immunised animals, suggesting an ability of Hu7ap to interact with or mimic autoantigenic macromolecules.",
keywords = "7ap proteins, Antinuclear antibodies, Nucleic acid binding features, ORF7a, Porcine reproductive and respiratory syndrome virus",
author = "F. Olasz and B. D{\'e}nes and A. B{\'a}lint and Tibor Magyar and S{\'a}ndor Belak and Z. Z{\'a}dori",
year = "2017",
month = "1",
day = "1",
doi = "10.1556/004.2017.013",
language = "English",
volume = "65",
pages = "124--134",
journal = "Acta Veterinaria Hungarica",
issn = "0236-6290",
publisher = "Akademiai Kiado",
number = "1",

}

TY - JOUR

T1 - Characterisation of the nucleic acid binding features of the PRRSV 7ap and its ability to induce antinuclear antibodies

AU - Olasz, F.

AU - Dénes, B.

AU - Bálint, A.

AU - Magyar, Tibor

AU - Belak, Sándor

AU - Zádori, Z.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - A short alternative open reading frame named ORF7a has recently been discovered within the nucleocapsid gene of the porcine reproductive and respiratory syndrome virus (PRRSV) genome. Proteins (7ap) translated from the ORF7a of two divergent strains -a type I and a type II -are able to completely reduce the motility of nucleic acids at relatively high molar charge ratios in gel retardation assays indicating strong dsDNA-and ssRNA-binding capability. Conserved RNAand DNA-binding properties suggest that nucleic acid binding is a functional property of the divergent 7aps, and not an arbitrary consequence of their net positive charge. Sera from Hu7ap-immunised pigs and mice did not react with Hu7ap or Hu7ap-GFP; however, antinuclear antibodies were detected in the sera of the immunised animals, suggesting an ability of Hu7ap to interact with or mimic autoantigenic macromolecules.

AB - A short alternative open reading frame named ORF7a has recently been discovered within the nucleocapsid gene of the porcine reproductive and respiratory syndrome virus (PRRSV) genome. Proteins (7ap) translated from the ORF7a of two divergent strains -a type I and a type II -are able to completely reduce the motility of nucleic acids at relatively high molar charge ratios in gel retardation assays indicating strong dsDNA-and ssRNA-binding capability. Conserved RNAand DNA-binding properties suggest that nucleic acid binding is a functional property of the divergent 7aps, and not an arbitrary consequence of their net positive charge. Sera from Hu7ap-immunised pigs and mice did not react with Hu7ap or Hu7ap-GFP; however, antinuclear antibodies were detected in the sera of the immunised animals, suggesting an ability of Hu7ap to interact with or mimic autoantigenic macromolecules.

KW - 7ap proteins

KW - Antinuclear antibodies

KW - Nucleic acid binding features

KW - ORF7a

KW - Porcine reproductive and respiratory syndrome virus

UR - http://www.scopus.com/inward/record.url?scp=85016213064&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85016213064&partnerID=8YFLogxK

U2 - 10.1556/004.2017.013

DO - 10.1556/004.2017.013

M3 - Article

C2 - 28244336

AN - SCOPUS:85016213064

VL - 65

SP - 124

EP - 134

JO - Acta Veterinaria Hungarica

JF - Acta Veterinaria Hungarica

SN - 0236-6290

IS - 1

ER -