Cellulase production using different streams of wheat grain- and wheat straw-based ethanol processes

Miklós Gyalai-Korpos, Réka Mangel, Pablo Alvira, Dóra Dienes, Mercedes Ballesteros, K. Réczey

Research output: Article

12 Citations (Scopus)

Abstract

Pretreatment is a necessary step in the biomass-to-ethanol conversion process. The side stream of the pretreatment step is the liquid fraction, also referred to as the hydrolyzate, which arises after the separation of the pretreated solid and is composed of valuable carbohydrates along with compounds that are potentially toxic to microbes (mainly furfural, acetic acid, and formic acid). The aim of our study was to utilize the liquid fraction from steam-exploded wheat straw as a carbon source for cellulase production by Trichoderma reesei RUT C30. Results showed that without detoxification, the fungus failed to utilize any dilution of the hydrolyzate; however, after a two-step detoxification process, it was able to grow on a fourfold dilution of the treated liquid fraction. Supplementation of the fourfold-diluted, treated liquid fraction with washed pretreated wheat straw or ground wheat grain led to enhanced cellulase (filter paper) activity. Produced enzymes were tested in hydrolysis of washed pretreated wheat straw. Supplementation with ground wheat grain provided a more efficient enzyme mixture for the hydrolysis by means of the near-doubled β-glucosidase activity obtained.

Original languageEnglish
Pages (from-to)791-802
Number of pages12
JournalJournal of Industrial Microbiology and Biotechnology
Volume38
Issue number7
DOIs
Publication statusPublished - júl. 2011

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Cellulase
Triticum
Ethanol
formic acid
Hydrolysis
Furaldehyde
Glucosidases
Trichoderma
Poisons
Steam
Enzymes
Acetic Acid
Biomass
Fungi
Carbon
Carbohydrates

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Cellulase production using different streams of wheat grain- and wheat straw-based ethanol processes. / Gyalai-Korpos, Miklós; Mangel, Réka; Alvira, Pablo; Dienes, Dóra; Ballesteros, Mercedes; Réczey, K.

In: Journal of Industrial Microbiology and Biotechnology, Vol. 38, No. 7, 07.2011, p. 791-802.

Research output: Article

Gyalai-Korpos, Miklós ; Mangel, Réka ; Alvira, Pablo ; Dienes, Dóra ; Ballesteros, Mercedes ; Réczey, K. / Cellulase production using different streams of wheat grain- and wheat straw-based ethanol processes. In: Journal of Industrial Microbiology and Biotechnology. 2011 ; Vol. 38, No. 7. pp. 791-802.
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