Cellular localisation of the proteins of region 3 of feline enteric coronavirus

I. Mészáros, F. Olasz, Enikő Kádár-Hürkecz, A. Bálint, A. Hornyák, Sándor Belák, Z. Zádori

Research output: Article

Abstract

Feline enteric coronaviruses have three open reading frames (ORFs) in region 3 (3a, 3b, and 3c). All three ORFs were expressed with C-terminal eGFP and 3xFLAG tags in different cell lines and their localisation was determined. ORF 3a is predicted to contain DNA-binding and transcription activator domains, and it is localised in the nucleus and in the cytoplasm. ORF 3b is also predicted to contain DNA-binding and activator domains, and was found to localise in the mitochondrion. Besides that, in some of the non-infected and FIPV-infected cells nucleolar, perinuclear or nuclear membrane accumulation of the eGFP-tagged 3b was observed. The exact compartmental localisation of ORF 3c is yet to be determined. However, based on our co-localisation studies 3c does not seem to be localised in the ER-Golgi network, ERGIC or peroxisomes. The expression of 3c-eGFP is clearly cell type dependent, it is more stable in MARC 145 cells than in Fcwf-4 or CrFK cells, which might reflect in vivo stability differences of 3c in natural target cells (enterocytes vs. monocytes/macrophages).

Original languageEnglish
Pages (from-to)493-508
Number of pages16
JournalActa Veterinaria Hungarica
Volume66
Issue number3
DOIs
Publication statusPublished - szept. 1 2018

Fingerprint

Feline Coronavirus
Feline coronavirus
Open Reading Frames
open reading frames
Proteins
proteins
cells
Peroxisomes
Enterocytes
DNA
Nuclear Envelope
enterocytes
nuclear membrane
peroxisomes
monocytes
Monocytes
Mitochondria
Cytoplasm
macrophages
mitochondria

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Cellular localisation of the proteins of region 3 of feline enteric coronavirus. / Mészáros, I.; Olasz, F.; Kádár-Hürkecz, Enikő; Bálint, A.; Hornyák, A.; Belák, Sándor; Zádori, Z.

In: Acta Veterinaria Hungarica, Vol. 66, No. 3, 01.09.2018, p. 493-508.

Research output: Article

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