Cellular factor XIII, a transglutaminase in human corneal keratocytes

Zsuzsanna Z. Orosz, Helga Bárdos, Amir H. Shemirani, Ildikó Beke Debreceni, Riitta Lassila, Antti S. Riikonen, Johanna A. Kremer Hovinga, Theo G. Seiler, Hendrika A. van Dorland, Verena Schroeder, Zoltán Boda, László Nemes, Beatrice Früh Eppstein, Bence Nagy, Andrea Facskó, János Kappelmayer, László Muszbek

Research output: Article

Abstract

Cellular factor XIII (cFXIII, FXIII-A2), a transglutaminase, has been demonstrated in a few cell types. Its main function is to cross-link proteins by isopeptide bonds. Here, we investigated the presence of cFXIII in cells of human cornea. Tissue sections of the cornea were immunostained for FXIII-A in combination with staining for CD34 antigen or isopeptide cross-links. Isolated corneal keratocytes were also evaluated by immunofluorescent microscopy and flow cytometry. FXIII-A in the corneal stroma was quantified by Western blotting. FXIII-A mRNA was detected by RT-qPCR. The cornea of FXIII-A-deficient patients was evaluated by cornea topography. FXIII-A was detected in 68 ± 13% of CD34+ keratocytes. Their distribution in the corneal stroma was unequal; they were most abundant in the subepithelial tertile. cFXIII was of cytoplasmic localization. In the stroma, 3.64 ng cFXIII/mg protein was measured. The synthesis of cFXIII by keratocytes was confirmed by RT-qPCR. Isopeptide cross-links were detected above, but not within the corneal stroma. Slight abnormality of the cornea was detected in six out of nine FXIII-A-deficient patients. The presence of cFXIII in human keratocytes was established for the first time. cFXIII might be involved in maintaining the stability of the cornea and in the corneal wound healing process.

Original languageEnglish
Article number5963
JournalInternational journal of molecular sciences
Volume20
Issue number23
DOIs
Publication statusPublished - dec. 2019

Fingerprint

Corneal Keratocytes
CD34 Antigens
Factor XIII
Transglutaminases
cornea
Flow cytometry
Cornea
Topography
Microscopic examination
Cells
Tissue
Proteins
Corneal Stroma
Messenger RNA
Antigens
proteins
wound healing
cytometry
abnormalities
antigens

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Orosz, Z. Z., Bárdos, H., Shemirani, A. H., Debreceni, I. B., Lassila, R., Riikonen, A. S., ... Muszbek, L. (2019). Cellular factor XIII, a transglutaminase in human corneal keratocytes. International journal of molecular sciences, 20(23), [5963]. https://doi.org/10.3390/ijms20235963

Cellular factor XIII, a transglutaminase in human corneal keratocytes. / Orosz, Zsuzsanna Z.; Bárdos, Helga; Shemirani, Amir H.; Debreceni, Ildikó Beke; Lassila, Riitta; Riikonen, Antti S.; Kremer Hovinga, Johanna A.; Seiler, Theo G.; van Dorland, Hendrika A.; Schroeder, Verena; Boda, Zoltán; Nemes, László; Eppstein, Beatrice Früh; Nagy, Bence; Facskó, Andrea; Kappelmayer, János; Muszbek, László.

In: International journal of molecular sciences, Vol. 20, No. 23, 5963, 12.2019.

Research output: Article

Orosz, ZZ, Bárdos, H, Shemirani, AH, Debreceni, IB, Lassila, R, Riikonen, AS, Kremer Hovinga, JA, Seiler, TG, van Dorland, HA, Schroeder, V, Boda, Z, Nemes, L, Eppstein, BF, Nagy, B, Facskó, A, Kappelmayer, J & Muszbek, L 2019, 'Cellular factor XIII, a transglutaminase in human corneal keratocytes', International journal of molecular sciences, vol. 20, no. 23, 5963. https://doi.org/10.3390/ijms20235963
Orosz ZZ, Bárdos H, Shemirani AH, Debreceni IB, Lassila R, Riikonen AS et al. Cellular factor XIII, a transglutaminase in human corneal keratocytes. International journal of molecular sciences. 2019 dec.;20(23). 5963. https://doi.org/10.3390/ijms20235963
Orosz, Zsuzsanna Z. ; Bárdos, Helga ; Shemirani, Amir H. ; Debreceni, Ildikó Beke ; Lassila, Riitta ; Riikonen, Antti S. ; Kremer Hovinga, Johanna A. ; Seiler, Theo G. ; van Dorland, Hendrika A. ; Schroeder, Verena ; Boda, Zoltán ; Nemes, László ; Eppstein, Beatrice Früh ; Nagy, Bence ; Facskó, Andrea ; Kappelmayer, János ; Muszbek, László. / Cellular factor XIII, a transglutaminase in human corneal keratocytes. In: International journal of molecular sciences. 2019 ; Vol. 20, No. 23.
@article{a3f409c9c9174f58bccff6050b9496dc,
title = "Cellular factor XIII, a transglutaminase in human corneal keratocytes",
abstract = "Cellular factor XIII (cFXIII, FXIII-A2), a transglutaminase, has been demonstrated in a few cell types. Its main function is to cross-link proteins by isopeptide bonds. Here, we investigated the presence of cFXIII in cells of human cornea. Tissue sections of the cornea were immunostained for FXIII-A in combination with staining for CD34 antigen or isopeptide cross-links. Isolated corneal keratocytes were also evaluated by immunofluorescent microscopy and flow cytometry. FXIII-A in the corneal stroma was quantified by Western blotting. FXIII-A mRNA was detected by RT-qPCR. The cornea of FXIII-A-deficient patients was evaluated by cornea topography. FXIII-A was detected in 68 ± 13{\%} of CD34+ keratocytes. Their distribution in the corneal stroma was unequal; they were most abundant in the subepithelial tertile. cFXIII was of cytoplasmic localization. In the stroma, 3.64 ng cFXIII/mg protein was measured. The synthesis of cFXIII by keratocytes was confirmed by RT-qPCR. Isopeptide cross-links were detected above, but not within the corneal stroma. Slight abnormality of the cornea was detected in six out of nine FXIII-A-deficient patients. The presence of cFXIII in human keratocytes was established for the first time. cFXIII might be involved in maintaining the stability of the cornea and in the corneal wound healing process.",
keywords = "Cornea, Factor XIII, Isopeptide bonds, Keratocytes, Transglutaminase",
author = "Orosz, {Zsuzsanna Z.} and Helga B{\'a}rdos and Shemirani, {Amir H.} and Debreceni, {Ildik{\'o} Beke} and Riitta Lassila and Riikonen, {Antti S.} and {Kremer Hovinga}, {Johanna A.} and Seiler, {Theo G.} and {van Dorland}, {Hendrika A.} and Verena Schroeder and Zolt{\'a}n Boda and L{\'a}szl{\'o} Nemes and Eppstein, {Beatrice Fr{\"u}h} and Bence Nagy and Andrea Facsk{\'o} and J{\'a}nos Kappelmayer and L{\'a}szl{\'o} Muszbek",
year = "2019",
month = "12",
doi = "10.3390/ijms20235963",
language = "English",
volume = "20",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "23",

}

TY - JOUR

T1 - Cellular factor XIII, a transglutaminase in human corneal keratocytes

AU - Orosz, Zsuzsanna Z.

AU - Bárdos, Helga

AU - Shemirani, Amir H.

AU - Debreceni, Ildikó Beke

AU - Lassila, Riitta

AU - Riikonen, Antti S.

AU - Kremer Hovinga, Johanna A.

AU - Seiler, Theo G.

AU - van Dorland, Hendrika A.

AU - Schroeder, Verena

AU - Boda, Zoltán

AU - Nemes, László

AU - Eppstein, Beatrice Früh

AU - Nagy, Bence

AU - Facskó, Andrea

AU - Kappelmayer, János

AU - Muszbek, László

PY - 2019/12

Y1 - 2019/12

N2 - Cellular factor XIII (cFXIII, FXIII-A2), a transglutaminase, has been demonstrated in a few cell types. Its main function is to cross-link proteins by isopeptide bonds. Here, we investigated the presence of cFXIII in cells of human cornea. Tissue sections of the cornea were immunostained for FXIII-A in combination with staining for CD34 antigen or isopeptide cross-links. Isolated corneal keratocytes were also evaluated by immunofluorescent microscopy and flow cytometry. FXIII-A in the corneal stroma was quantified by Western blotting. FXIII-A mRNA was detected by RT-qPCR. The cornea of FXIII-A-deficient patients was evaluated by cornea topography. FXIII-A was detected in 68 ± 13% of CD34+ keratocytes. Their distribution in the corneal stroma was unequal; they were most abundant in the subepithelial tertile. cFXIII was of cytoplasmic localization. In the stroma, 3.64 ng cFXIII/mg protein was measured. The synthesis of cFXIII by keratocytes was confirmed by RT-qPCR. Isopeptide cross-links were detected above, but not within the corneal stroma. Slight abnormality of the cornea was detected in six out of nine FXIII-A-deficient patients. The presence of cFXIII in human keratocytes was established for the first time. cFXIII might be involved in maintaining the stability of the cornea and in the corneal wound healing process.

AB - Cellular factor XIII (cFXIII, FXIII-A2), a transglutaminase, has been demonstrated in a few cell types. Its main function is to cross-link proteins by isopeptide bonds. Here, we investigated the presence of cFXIII in cells of human cornea. Tissue sections of the cornea were immunostained for FXIII-A in combination with staining for CD34 antigen or isopeptide cross-links. Isolated corneal keratocytes were also evaluated by immunofluorescent microscopy and flow cytometry. FXIII-A in the corneal stroma was quantified by Western blotting. FXIII-A mRNA was detected by RT-qPCR. The cornea of FXIII-A-deficient patients was evaluated by cornea topography. FXIII-A was detected in 68 ± 13% of CD34+ keratocytes. Their distribution in the corneal stroma was unequal; they were most abundant in the subepithelial tertile. cFXIII was of cytoplasmic localization. In the stroma, 3.64 ng cFXIII/mg protein was measured. The synthesis of cFXIII by keratocytes was confirmed by RT-qPCR. Isopeptide cross-links were detected above, but not within the corneal stroma. Slight abnormality of the cornea was detected in six out of nine FXIII-A-deficient patients. The presence of cFXIII in human keratocytes was established for the first time. cFXIII might be involved in maintaining the stability of the cornea and in the corneal wound healing process.

KW - Cornea

KW - Factor XIII

KW - Isopeptide bonds

KW - Keratocytes

KW - Transglutaminase

UR - http://www.scopus.com/inward/record.url?scp=85075691929&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85075691929&partnerID=8YFLogxK

U2 - 10.3390/ijms20235963

DO - 10.3390/ijms20235963

M3 - Article

C2 - 31783511

AN - SCOPUS:85075691929

VL - 20

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 23

M1 - 5963

ER -