Cell-cycle, phase-specific activation of Maize streak virus promoters

K. Nikovics, J. Simidjieva, A. Peres, F. Ayaydin, T. Pasternak, J. W. Davies, M. I. Boulton, D. Dudits, G. Horváth

Research output: Article

8 Citations (Scopus)

Abstract

It is believed that geminiviral DNA replication is coupled to the cell-cycle regulatory complex of the plant cell and that the virus-early (complementary or C sense) gene products REP and REPA may be able to manipulate the regulation of the cycle. In this study, we examined expression from the promoters of Maize streak virus (MSV) in transgenic maize plants and cells to determine whether they showed cell-cycle specificity. Histochemical staining of plant roots containing "long and short" C-sense promoter sequences upstream of the GUS (β-glucuronidase) reporter gene showed that promoter activity was restricted to the meristematic region of the roots and was enhanced by 2,4-dichlorophenoxy acetic acid (2,4-D) treatment. Analysis of reporter gene and cell-cycle-specific gene transcript levels coupled with flow cytometric data in synchronized transgenic maize cells revealed that all of the MSV promoters showed cell-cycle specificity. The coat protein gene promoter showed highest activity in early G2, whereas the C-sense promoter sequences produced two peaks of activity in the S and G2 cell-cycle phases.

Original languageEnglish
Pages (from-to)609-617
Number of pages9
JournalMolecular Plant-Microbe Interactions
Volume14
Issue number5
Publication statusPublished - 2001

Fingerprint

Maize streak virus
Viruses
cell cycle
Cell Cycle
Genes
Chemical activation
Cells
promoter regions
2,4-Dichlorophenoxyacetic Acid
Plant Cells
Reporter Genes
Zea mays
Plant Viruses
reporter genes
cdc Genes
2,4-D
Plant Roots
Genetically Modified Plants
Glucuronidase
Capsid Proteins

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

Cite this

Nikovics, K., Simidjieva, J., Peres, A., Ayaydin, F., Pasternak, T., Davies, J. W., ... Horváth, G. (2001). Cell-cycle, phase-specific activation of Maize streak virus promoters. Molecular Plant-Microbe Interactions, 14(5), 609-617.

Cell-cycle, phase-specific activation of Maize streak virus promoters. / Nikovics, K.; Simidjieva, J.; Peres, A.; Ayaydin, F.; Pasternak, T.; Davies, J. W.; Boulton, M. I.; Dudits, D.; Horváth, G.

In: Molecular Plant-Microbe Interactions, Vol. 14, No. 5, 2001, p. 609-617.

Research output: Article

Nikovics, K, Simidjieva, J, Peres, A, Ayaydin, F, Pasternak, T, Davies, JW, Boulton, MI, Dudits, D & Horváth, G 2001, 'Cell-cycle, phase-specific activation of Maize streak virus promoters', Molecular Plant-Microbe Interactions, vol. 14, no. 5, pp. 609-617.
Nikovics K, Simidjieva J, Peres A, Ayaydin F, Pasternak T, Davies JW et al. Cell-cycle, phase-specific activation of Maize streak virus promoters. Molecular Plant-Microbe Interactions. 2001;14(5):609-617.
Nikovics, K. ; Simidjieva, J. ; Peres, A. ; Ayaydin, F. ; Pasternak, T. ; Davies, J. W. ; Boulton, M. I. ; Dudits, D. ; Horváth, G. / Cell-cycle, phase-specific activation of Maize streak virus promoters. In: Molecular Plant-Microbe Interactions. 2001 ; Vol. 14, No. 5. pp. 609-617.
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