Capsaicin-Sensitive Sensory Nerves Mediate the Cellular and Microvascular Effects of H2S via TRPA1 Receptor Activation and Neuropeptide Release

Zsófia Hajna, Éva Sághy, Maja Payrits, Aisah A. Aubdool, Éva Szőke, Gábor Pozsgai, István Z. Bátai, Lívia Nagy, Dániel Filotás, Zsuzsanna Helyes, Susan D. Brain, Erika Pintér

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Abstract

It is supposed that TRPA1 receptor can be activated by hydrogen sulphide (H2S). Here, we have investigated the role of TRPA1 receptor in H2S-induced [Ca2+]i increase in trigeminal ganglia (TRG) neurons, and the involvement of capsaicin-sensitive sensory nerves in H2S-evoked cutaneous vasodilatation. [Ca2+]i was measured with ratiometric technique on TRG neurons of TRPA1+/+ and TRPA1−/− mice after NaHS, Na2S, allylisothiocyanate (AITC) or KCl treatment. Microcirculatory changes in the ear were detected by laser Doppler imaging in response to topical NaHS, AITC, NaOH, NaSO3 or NaCl. Mice were either treated with resiniferatoxin (RTX), or CGRP antagonist BIBN4096, or NK1 receptor antagonist CP99994, or K+ ATP channel blocker glibenclamide. Alpha-CGRP−/− and NK1 −/− mice were also investigated. NaHS and Na2S increased [Ca2+]i in TRG neurons derived from TRPA+/+ but not from TRPA1−/− mice. NaHS increased cutaneous blood flow, while NaOH, NaSO3 and NaCl did not cause significant changes. NaHS-induced vasodilatation was reduced in RTX-treated animals, as well as by pre-treatment with BIBN4096 or CP99994 alone or in combination. NaHS-induced vasodilatation was significantly smaller in alpha-CGRP−/− or NK1 −/− mice compared to wild-types. H2S activates capsaicin-sensitive sensory nerves through TRPA1 receptors and the resultant vasodilatation is mediated by the release of vasoactive sensory neuropeptides CGRP and substance P.

Original languageEnglish
Pages (from-to)157-170
Number of pages14
JournalJournal of Molecular Neuroscience
Volume60
Issue number2
DOIs
Publication statusPublished - okt. 1 2016

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ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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